Rats Exposed to Excess Sucrose During a Critical Period Develop Inflammation and Express a Secretory Phenotype of Vascular Smooth Muscle Cells.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-10-17 DOI:10.3390/metabo14100555

Verónica Guarner-Lans, Elizabeth Soria-Castro, Agustina Cano-Martínez, María Esther Rubio-Ruiz, Gabriela Zarco, Elizabeth Carreón-Torres, Oscar Grimaldo, Vicente Castrejón-Téllez, Israel Pérez-Torres

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引用次数: 0

Abstract

Background: Neonatal rats that receive sucrose during a critical postnatal period (CP, days 12 to 28) develop hypertension by the time they reach adulthood. Inflammation might contribute to changes during this period and could be associated with variations in the vascular smooth muscle (VSMC) phenotype.

Objective: We studied changes in inflammatory pathways that could underlie the expression of the secretory phenotype in the VSMC in the thoracic aorta of rats that received sucrose during CP.

Methods: We analyzed histological changes in the aorta and the expression of the COX-2, TLR4, iNOS, eNOS, MMP-2 and -9, and β- and α-actin, the quantities of TNF-α, IL-6, and IL-1β using ELISA, and the levels of fatty acids using gas chromatography.

Results: The aortic wall presented disorganization, decellularization, and wavy elastic fibers and an increase in the lumen area. The α- and β-actin expressions were decreased, while COX-2, TLR4, TNF-α, and the activity of IL-6 were increased. Oleic acid was increased in CP in comparison to the control group.

Conclusions: There is transient hypertension at the end of the CP that is accompanied by inflammation and a change in the phenotype of VSMC to the secretory phenotype. The inflammatory changes could act as epigenetic signals to determine the development of hypertension when animals reach adulthood.

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在关键时期暴露于过量蔗糖的大鼠会出现炎症并表达血管平滑肌细胞的分泌型。

背景：在出生后的关键时期（CP，第 12 至 28 天）接受蔗糖的新生大鼠在成年后会患高血压。炎症可能会导致这一时期的变化，并可能与血管平滑肌（VSMC）表型的变化有关：我们研究了炎症通路的变化，这些变化可能是CP期间摄入蔗糖的大鼠胸主动脉血管平滑肌（VSMC）分泌表型表达的基础：我们分析了大鼠主动脉的组织学变化以及 COX-2、TLR4、iNOS、eNOS、MMP-2 和 -9、β- 和 α-肌动蛋白的表达，使用 ELISA 分析了 TNF-α、IL-6 和 IL-1β 的含量，使用气相色谱分析了脂肪酸的含量：结果：主动脉壁出现紊乱、脱细胞和波浪状弹性纤维，管腔面积增大。α-和β-肌动蛋白的表达减少，而COX-2、TLR4、TNF-α和IL-6的活性增加。与对照组相比，CP 组油酸增加：结论：CP 结束时会出现一过性高血压，伴随着炎症和 VSMC 表型向分泌表型的转变。炎症变化可作为表观遗传学信号，决定动物成年后高血压的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.