BW312 Hordeum vulgare semi-dwarf mutant exhibits a shifted metabolic profile towards pathogen resistance.

IF 3.5 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Richard Rigo, Julie Zumsteg, Hubert Schaller, Thierry Barchietto, Sergej Buchet, Dimitri Heintz, Claire Villette
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Abstract

Introduction: Plant hormonal mutants, which do not produce or are insensitive to hormones, are often affected in their growth and development, but other metabolic rearrangements might be involved. A trade-off between growth and stress response is necessary for the plant survival.

Objectives: Here, we explore the metabolic profile and the pathogen resistance of a brassinosteroid-insensitive Hordeum vulgare L. semi-dwarf mutant, BW312.

Methods: We investigate BW312 metabolism through a chemical enrichment analysis, confirming a shifted metabolic profile towards pathogen resistance. The effective pathogen resistance of the mutant was tested in presence of Pyrenophora teres and Fusarium graminearum.

Results: Four compound families were increased in the mutant (pyrrolidines, basic amino acids, alkaloids, monounsaturated fatty acids), while two compound families were decreased (pyrrolidinones, anthocyanins). Dipeptides were also altered (increased and decreased). BW312 displayed a better resistance to Pyrenophora teres in the earliest stage of infection with a 21.5% decrease of the lesion length 10 days after infection. BW312 also exhibited a reduced lesion length (43.3%) and a reduced browning of the lesions (55.5%) when exposed to Fusarium graminearum at the seedling stage.

Conclusion: The observed metabolomic shift strongly suggests that the BW312 semi-dwarf mutant is in a primed state, resulting in a standby state of alertness to pathogens.

BW312 Hordeum vulgare 半矮小突变体表现出抗病原体转移的代谢特征。
导言:植物激素突变体不产生激素或对激素不敏感,通常会影响其生长和发育,但也可能涉及其他代谢重新排列。植物的生存需要在生长和胁迫反应之间做出权衡:在此,我们探讨了对黄铜类固醇不敏感的半矮小突变体 Hordeum vulgare L. BW312 的代谢概况和病原体抗性:我们通过化学富集分析研究了 BW312 的新陈代谢,证实其新陈代谢特征已转向抗病原性。在赤霉病菌和禾谷镰刀菌存在的情况下,测试了突变体对病原体的有效抗性:结果:突变体中的四个化合物家族(吡咯烷酮、碱性氨基酸、生物碱、单不饱和脂肪酸)有所增加,而两个化合物家族(吡咯烷酮、花青素)有所减少。二肽也发生了变化(增加和减少)。在感染初期,BW312 对赤霉病有更好的抗性,感染 10 天后,病斑长度减少了 21.5%。BW312 在幼苗期受到禾本科镰刀菌感染时,也表现出病害长度减少(43.3%)和病害褐变减少(55.5%):观察到的代谢组变化强烈表明,BW312 半矮小突变体处于引诱状态,导致其对病原体的警觉性处于待机状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metabolomics
Metabolomics 医学-内分泌学与代谢
CiteScore
6.60
自引率
2.80%
发文量
84
审稿时长
2 months
期刊介绍: Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.
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