Ultra-High-Performance Liquid Chromatography-High-Definition Mass Spectrometry-Based Metabolomics to Reveal the Potential Anti-Arthritic Effects of Illicium verum in Cultured Fibroblast-like Synoviocytes Derived from Rheumatoid Arthritis.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-09-25 DOI:10.3390/metabo14100517

Mingzhen Qin, Lu Chen, Xiaoli Hou, Wuwei Wu, Yu Liu, Yu Pan, Mengli Zhang, Zhien Tan, Danna Huang

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引用次数: 0

Abstract

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits of Illicium verum, which is a medicinal and edible resource, have been shown to have anti-inflammatory properties.

Methods: In this study, we investigated the effects of I. verum extracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method. We subsequently analyzed the metabolites produced after the incubation of cultured RA-FLS with IVEs.

Results: IVEs inhibited the proliferation and suppressed the migration of RA-FLS, and reduced the levels of inflammatory factors including TNF-α and IL-6. Twenty differential metabolites responsible for the effects of IVEs were screened and annotated based on the UPLC-HDMS data by using a cell metabolomics approach.

Discussion: Our findings suggest that treating RA-FLS with IVEs can regulate lipid and amino acid metabolism, indicating that this extract has the potential to modify the metabolic pathways that cause inflammation in RA.

Conclusions: This might lead to novel therapeutic strategies for managing patients with RA.

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基于超高效液相色谱-高分辨质谱联用技术的代谢组学研究揭示茵陈在类风湿性关节炎纤维母细胞样滑膜培养细胞中的潜在抗关节炎作用

背景：类风湿性关节炎（RA）是一种慢性炎症性自身免疫疾病。作为药用和食用资源的茵陈果实已被证明具有抗炎特性：在这项研究中，我们采用灵敏且选择性高的超高效液相色谱-高清质谱（UPLC-HDMS）方法，研究了茵陈提取物（IVEs）对人RA成纤维细胞样滑膜细胞（RA-FLS）的影响。我们随后分析了用 IVEs 培养 RA-FLS 后产生的代谢物：结果：IVEs抑制了RA-FLS的增殖和迁移，降低了TNF-α和IL-6等炎症因子的水平。讨论：我们的研究结果表明，IVEs能抑制RA-FLS的增殖和迁移，降低TNF-α和IL-6等炎症因子的水平：讨论：我们的研究结果表明，用IVEs治疗RA-FLS可调节脂质和氨基酸代谢，表明这种提取物有可能改变导致RA炎症的代谢途径：结论：这可能会为治疗 RA 患者带来新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.