Effects of Different Photoperiods on Peripheral 5-Hydroxytryptamine Metabolism, Breast Muscle Glucose Metabolism, and Myopathies in Broilers.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2024-10-21 DOI:10.3390/metabo14100567
Miao Yu, Mengjie Xu, Guangju Wang, Jinghai Feng, Minhong Zhang
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引用次数: 0

Abstract

Background: There is a close relationship between breast muscle glucose metabolism, peripheral 5-hydroxytryptamine (5-HT), and myopathies in animals. Here, this study aimed to investigate the effects of different photoperiods on peripheral 5-HT metabolism, white striping (WS), and wooden breast (WB) in broilers. Methods: A total of 216 healthy 5-day-old (d) Arbor Acres (AA) male broilers were randomly assigned to 12L:12D, 18L:6D, and 24L:0D photoperiods for 4 weeks. Results: Compared with the 12L:12D photoperiod, we found the WB score in broilers was significantly increased in the 18L:6D and 24L:0D photoperiod at week 4 (p < 0.05). Muscle glycogen was significantly reduced (p < 0.05) and glycolysis was promoted in the breast muscles of broilers under the 18L:6D and 24L:0D photoperiods at week 2 and 4. Peripheral 5-HT concentrations, the mRNA expression of tryptophan hydroxylase 1 (TPH1) and serotonin transporter (SERT) in the cecal mucosa, and 5-hydroxytryptamine receptor 2A (5-HTR2A) mRNA expression in the breast muscle of broilers significantly up-regulated in the 18L:6D and 24L:0D photoperiod at week 2 and 4 (p < 0.05). Conclusions: Our findings revealed that extending the photoperiod improved the breast muscle growth rate, but up-regulated 5-HT synthesis and secretion to higher peripheral 5-HT, induced breast muscle glucose metabolism disorder, and increased WB incidence rates in broilers.

不同光周期对肉鸡外周5-羟色胺代谢、胸肌葡萄糖代谢和肌病的影响
背景:乳房肌肉葡萄糖代谢、外周5-羟色胺(5-HT)和动物肌病之间存在密切关系。本研究旨在探讨不同光周期对肉鸡外周5-羟色胺代谢、白条纹(WS)和木胸(WB)的影响。研究方法将 216 只健康的 5 日龄(d)Arbor Acres(AA)雄性肉鸡随机分配到 12L:12D、18L:6D 和 24L:0D 光周期下饲养 4 周。结果:与 12L:12D 光周期相比,我们发现肉鸡的 WB 评分在 18L:6D 和 24L:0D 光周期的第 4 周显著增加(p < 0.05)。在第 2 周和第 4 周,18L:6D 和 24L:0D 光周期下肉鸡胸肌中的肌糖原明显减少(p < 0.05),糖酵解被促进。外周5-HT浓度、色氨酸羟化酶1(TPH1)和5-羟色胺转运体(SERT)在盲肠粘膜中的mRNA表达以及5-羟色胺受体2A(5-HTR2A)在第2周和第4周肉鸡胸肌中的mRNA表达在18L:6D和24L:0D光周期下显著上调(p < 0.05)。结论我们的研究结果表明,延长光周期可提高肉鸡胸肌的生长速度,但会上调 5-HT 的合成和分泌,使外周 5-HT 水平升高,诱发胸肌糖代谢紊乱,并增加 WB 的发病率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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