Proteomic Analysis and Biochemical Characterization of the Nematocyst Extract of the Hydrozoan Velella velella.

IF 4.9 2区 医学 Q1 CHEMISTRY, MEDICINAL
Marine Drugs Pub Date : 2024-10-12 DOI:10.3390/md22100468
Eleonora Tassara, Ivan Mikšík, Petr Pompach, Gian Luigi Mariottini, Liang Xiao, Marco Giovine, Marina Pozzolini
{"title":"Proteomic Analysis and Biochemical Characterization of the Nematocyst Extract of the Hydrozoan <i>Velella velella</i>.","authors":"Eleonora Tassara, Ivan Mikšík, Petr Pompach, Gian Luigi Mariottini, Liang Xiao, Marco Giovine, Marina Pozzolini","doi":"10.3390/md22100468","DOIUrl":null,"url":null,"abstract":"<p><p>The venom contained within cnidarian nematocysts has a complex composition and holds significant potential for biotechnological applications. In this context, one of the most effective methods for studying nematocyst contents is the proteomic approach, which can detect even trace amounts of compounds while minimizing the need for large-scale animal collection, thus helping to preserve ecosystem integrity. This study aimed to provide a comprehensive proteomic and biochemical characterization of the crude nematocyst extract from the common hydrozoan <i>Velella velella</i>. Despite not being harmful to humans, the analysis of the crude venom extract from <i>V. velella</i> brought to the identification of 783 different proteins, categorized into structural components, enzymes, and potential toxins, revealing a qualitative composition of the venom similar to that of other more toxic cnidarians. Biochemical assays confirmed the presence of various active hydrolytic enzymes within the extract, including proteases, phospholipases, hyaluronidases, DNases, and chitinases. These findings pave the road for future studies involving the pharmacological applications of <i>Velella velella</i> venom components through recombinant production and functional testing.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509761/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Drugs","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/md22100468","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0

Abstract

The venom contained within cnidarian nematocysts has a complex composition and holds significant potential for biotechnological applications. In this context, one of the most effective methods for studying nematocyst contents is the proteomic approach, which can detect even trace amounts of compounds while minimizing the need for large-scale animal collection, thus helping to preserve ecosystem integrity. This study aimed to provide a comprehensive proteomic and biochemical characterization of the crude nematocyst extract from the common hydrozoan Velella velella. Despite not being harmful to humans, the analysis of the crude venom extract from V. velella brought to the identification of 783 different proteins, categorized into structural components, enzymes, and potential toxins, revealing a qualitative composition of the venom similar to that of other more toxic cnidarians. Biochemical assays confirmed the presence of various active hydrolytic enzymes within the extract, including proteases, phospholipases, hyaluronidases, DNases, and chitinases. These findings pave the road for future studies involving the pharmacological applications of Velella velella venom components through recombinant production and functional testing.

水螅 Velella velella 的线虫囊提取物的蛋白质组分析和生化特征。
刺网动物线虫囊中所含的毒液成分复杂,具有生物技术应用的巨大潜力。在这种情况下,研究线虫囊内容物最有效的方法之一是蛋白质组学方法,它可以检测即使是痕量的化合物,同时最大限度地减少大规模动物采集的需要,从而有助于保护生态系统的完整性。本研究旨在对普通水螅 Velella velella 的粗线虫囊提取物进行全面的蛋白质组学和生化鉴定。尽管 V. velella 的毒液提取物对人体无害,但通过对其粗毒液提取物的分析,发现了 783 种不同的蛋白质,分为结构成分、酶和潜在毒素,揭示了毒液的质量组成与其他毒性更强的刺胞动物类似。生化检测证实了提取物中存在各种活性水解酶,包括蛋白酶、磷脂酶、透明质酸酶、脱氧核糖核酸酶和几丁质酶。这些发现为今后通过重组生产和功能测试研究 Velella velella 毒液成分的药理应用铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Marine Drugs
Marine Drugs 医学-医药化学
CiteScore
9.60
自引率
14.80%
发文量
671
审稿时长
1 months
期刊介绍: Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信