Phycocyanin-Loaded Alginate-Based Hydrogel Synthesis and Characterization.

IF 4.9 2区 医学 Q1 CHEMISTRY, MEDICINAL
Marine Drugs Pub Date : 2024-09-25 DOI:10.3390/md22100434
Diana-Ioana Buliga, Alexandra Mocanu, Edina Rusen, Aurel Diacon, Gabriela Toader, Oana Brincoveanu, Ioan Călinescu, Aurelian Cristian Boscornea
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Abstract

Phycocyanin was extracted from Spirulina platensis using conventional extraction (CE), direct ultrasonic-assisted extraction (direct UAE), indirect ultrasonic-assisted extraction (indirect UAE), and microwave-assisted extraction (MAE) methods at different temperatures, extraction intervals, stirring rate, and power intensities while maintaining the same algae to solvent ratio (1:15 w/v). The optimization of the extraction parameters indicated that the direct UAE yielded the highest phycocyanin concentration (29.31 ± 0.33 mg/mL) and antioxidant activity (23.6 ± 0.56 mg TE/g algae), while MAE achieved the highest purity (Rp = 0.5 ± 0.002). Based on the RP value, phycocyanin extract obtained by MAE (1:15 w/v algae to solvent ratio, 40 min, 40 °C, and 900 rpm) was selected as active compound in an alginate-based hydrogel formulation designed as potential wound dressings. Phycocyanin extracts and loaded hydrogels were characterized by FT-IR analysis. SEM analysis confirmed a porous structure for both blank and phycocyanin loaded hydrogels, while the mechanical properties remained approximately unchanged in the presence of phycocyanin. Phycocyanin release kinetics was investigated at two pH values using Zero-order, First-order, Higuchi, and Korsmeyer-Peppas kinetics models. The Higuchi model best fitted the experimental results. The R2 value at higher pH was nearly 1, indicating a superior fit compared with lower pH values.

植物花青素负载藻酸盐水凝胶的合成与表征。
在保持相同的藻类与溶剂比(1:15 w/v)的条件下,采用常规萃取法(CE)、超声波辅助直接萃取法(UAE)、超声波辅助间接萃取法(UAE)和微波辅助萃取法(MAE),在不同的温度、萃取间隔、搅拌速率和功率强度下萃取板蓝根螺旋藻中的植物花青素。萃取参数的优化结果表明,直接 UAE 法提取的藻蓝蛋白浓度(29.31 ± 0.33 mg/mL)和抗氧化活性(23.6 ± 0.56 mg TE/g)最高,而 MAE 法提取的藻蓝蛋白纯度(Rp = 0.5 ± 0.002)最高。根据 RP 值,MAE 提取的植物花青素(海藻与溶剂的比例为 1:15,40 分钟,40 °C,900 转/分)被选为藻酸盐水凝胶配方中的活性化合物,该配方被设计为潜在的伤口敷料。傅立叶变换红外分析对植物花青素提取物和负载水凝胶进行了表征。扫描电镜分析证实,空白水凝胶和含有植物花青素的水凝胶都具有多孔结构,而机械性能在含有植物花青素的情况下基本保持不变。使用零阶、一阶、Higuchi 和 Korsmeyer-Peppas 动力学模型研究了两种 pH 值下的植物花青素释放动力学。Higuchi 模型最符合实验结果。较高 pH 值下的 R2 值接近 1,表明其拟合效果优于较低 pH 值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Marine Drugs
Marine Drugs 医学-医药化学
CiteScore
9.60
自引率
14.80%
发文量
671
审稿时长
1 months
期刊介绍: Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.
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