Anatomy and Immunohistochemistry of Woodpecker Tail Muscles

Abstract

Woodpeckers (Order Piciformes) belong to a group of birds characterized by their hammering capabilities in which the bill is utilized as a tool to probe for food and to excavate nest cavities. They have numerous specializations for this behavior, including their bill and tongue, feet for gripping vertical tree trunks, and tail feathers with thickened shafts to provide stability as a postural appendage. We hypothesized that (1) woodpecker tail musculature is also modified for clinging behaviors with a heterogeneous distribution of fast and slow muscle fibers, and that (2) the tree-trunk foraging Hairy Woodpeckers would have more slow muscle fibers in their M. depressor caudae than Northern Flickers, which forage on the ground where they probe the substrate for insects. We performed immunohistochemistry to identify the fiber type distributions for tail muscles Mm. caudofemoralis pars caudalis, lateralis caudae, levator caudae, and depressor caudae in four Hairy Woodpeckers and five Northern Flickers. Our results show that these tail muscles in the two woodpecker species are comprised of a majority of fast muscle fibers common among dynamic locomotor muscles. Interestingly, we report a functionally-significant distribution of slow muscle fibers in M. depressor caudae predicted to be utilized in propping of the tail during tree climbing and support. Further, we found more slow fibers (13.80% ± 4.49%) in the trunk-foraging Hairy Woodpeckers compared with the ground-foraging Northern Flicker (7.40% ± 4.95%), which we interpret to be related to the trunk-foraging habits of Hairy Woodpeckers.