Continuous Global Improvement of Human Papillomavirus (HPV) Genotyping Services: The 2022 and 2023 HPV LabNet International Proficiency Studies

IF 6.8 3区 医学 Q1 VIROLOGY
Laila Sara Arroyo Mühr, Carina Eklund, Camilla Lagheden, Emel Yilmaz, Ola Forslund, Marina Lilja, Joakim Dillner
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Abstract

The International Human Papillomavirus (HPV) Reference Center launches annual global HPV genotyping proficiency panels to enhance the precision and international standardization of HPV genotyping services. This study aims to assess the proficiency levels achieved in the global HPV genotyping proficiency panels conducted in 2022 and 2023, and to evaluate trends in performance over time. The proficiency panels comprised 44 blinded samples each, including 40 samples containing various purified plasmids corresponding to HPV types combined with human DNA, plus four control samples (one negative control and three extraction controls). Proficiency required a sensitivity of 50 International Units (IU)/5 µL for HPV 16 and HPV 18 500 IU/5 µL for HPVs 6, 11, 31, 33, 45, 52, and 58 and 500 genome equivalents (GE)/5 µL for other HPV types in both single and multiple infections, while avoiding false positivity. In 2022, 78 laboratories submitted a total of 154 data sets, and in 2023, 81 laboratories contributed 141 data sets. Most data sets (87%, 258/295) utilized commercially available HPV assays. Proficiency was common, with 77% of data sets meeting the proficiency criteria in 2022 and 79% in 2023. False positive results significantly decreased from 22% in 2022 to 13% in 2023. The high proficiency and increasing specificity in HPV genotyping services indicates progress toward more reliable HPV testing. High accuracy is crucial for supporting global efforts in HPV and cervical cancer elimination.

全球人类乳头瘤病毒 (HPV) 基因分型服务的持续改进:2022 年和 2023 年 HPV LabNet 国际能力研究。
国际人乳头瘤病毒(HPV)参考中心每年都会推出全球HPV基因分型能力验证,以提高HPV基因分型服务的精确性和国际标准化。本研究旨在评估2022年和2023年进行的全球HPV基因分型能力验证所达到的能力水平,并评估随时间推移的能力趋势。能力验证小组由 44 份盲法样本组成,其中 40 份样本含有与人类 DNA 结合的 HPV 类型相对应的各种纯化质粒,另外还有 4 份对照样本(1 份阴性对照和 3 份提取对照)。对于 HPV 16 和 HPV 18,检测灵敏度要求为 50 国际单位 (IU)/5 µL ;对于 HPV 6、11、31、33、45、52 和 58,检测灵敏度要求为 500 国际单位/5 µL ;对于其他 HPV 类型,检测灵敏度要求为 500 基因组当量 (GE)/5 µL (包括单次感染和多次感染),同时避免假阳性。2022 年,78 家实验室共提交了 154 组数据;2023 年,81 家实验室提交了 141 组数据。大多数数据集(87%,258/295)采用了市售的 HPV 检测方法。熟练度很普遍,2022 年有 77% 的数据集符合熟练度标准,2023 年有 79% 的数据集符合熟练度标准。假阳性结果从 2022 年的 22% 显著下降到 2023 年的 13%。HPV基因分型服务的高熟练度和不断提高的特异性表明,在进行更可靠的HPV检测方面取得了进展。高准确性对于支持全球消除人乳头瘤病毒和宫颈癌的努力至关重要。
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来源期刊
Journal of Medical Virology
Journal of Medical Virology 医学-病毒学
CiteScore
23.20
自引率
2.40%
发文量
777
审稿时长
1 months
期刊介绍: The Journal of Medical Virology focuses on publishing original scientific papers on both basic and applied research related to viruses that affect humans. The journal publishes reports covering a wide range of topics, including the characterization, diagnosis, epidemiology, immunology, and pathogenesis of human virus infections. It also includes studies on virus morphology, genetics, replication, and interactions with host cells. The intended readership of the journal includes virologists, microbiologists, immunologists, infectious disease specialists, diagnostic laboratory technologists, epidemiologists, hematologists, and cell biologists. The Journal of Medical Virology is indexed and abstracted in various databases, including Abstracts in Anthropology (Sage), CABI, AgBiotech News & Information, National Agricultural Library, Biological Abstracts, Embase, Global Health, Web of Science, Veterinary Bulletin, and others.
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