Eco-Friendly Analytical Approach for Sensitive Spectrofluorimetric Determination of the Flavonoid Chrysin in Capsules and Human Plasma.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Heba Abd El-Aziz, Nada E Hammouda, Fathallah Belal, Heba Samir Elama
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Abstract

Chrysin is a plant flavonoid that has different therapeutic effects as anti-inflammatory, anti-cancer, anti-oxidant, and immune booster. Spectrofluorimetry has received a lot of interest lately because of its ecological greenness and analytical performance. This approach employed the native fluorescence of chrysin at 339 nm following excitation at 231 nm in distilled water. Modern advances in analytical chemistry have been used to lessen occupational and environmental concerns by employing distilled water as a dilution solvent through method development and application. The approach was found to be excellent green supported by eco-scale score of 97 and 0.94 AGREE rating, in addition to an overall whiteness score of 88.80. The design aimed to analyze chrysin in raw materials, Chrysin® capsules and human plasma. The method was linear over 0.5-7.0 ng mL⁻1 chrysin, with LOD of 0.06 ng mL⁻1 and LOQ of 0.20 ng mL⁻1. The offered method was effectively applied for determination of chrysin in the commercial capsules Chrysin® and spiked human plasma samples with average recoveries of 99.76% and 99.98%, respectively for capsules and spiked human plasma. Up to date, no spectrofluorimetric method has been described for chrysin analysis, then, this presented an opportunity to develop a sensitive, quick, reliable, environmentally friendly, and valid fluorescence-based method.

用生态友好型分析方法灵敏测定胶囊和人体血浆中的黄酮类化合物菊黄素
菊黄素是一种植物黄酮类化合物,具有消炎、抗癌、抗氧化和增强免疫力等不同的治疗效果。光谱荧光测定法因其绿色生态和分析性能而受到广泛关注。这种方法利用了蒸馏水中 231 纳米波长激发后 339 纳米波长处菊黄素的原生荧光。通过方法的开发和应用,利用蒸馏水作为稀释溶剂,现代分析化学的进步减少了职业和环境问题。结果表明,除了总体白度得分为 88.80 外,该方法的生态尺度得分为 97,AGREE 评分为 0.94,是一种出色的绿色方法。该设计旨在分析原材料、Chrysin®胶囊和人体血浆中的菊黄素。该方法在0.5-7.0 ng mL-1浓度范围内线性关系良好,最低检出限为0.06 ng mL-1,最低定量限为0.20 ng mL-1。该方法有效地测定了Chrysin®商品胶囊和加标人体血浆样品中的菊黄素含量,胶囊和加标人体血浆的平均回收率分别为99.76%和99.98%。迄今为止,还没有一种光谱荧光法可用于蛹虫草苷的分析,这为开发一种灵敏、快速、可靠、环保和有效的荧光法提供了机会。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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