Design and In Vitro Evaluation of Fluorescent MOF-Core CaCO₃-PEI-FA Shell Nanoparticles for Targeted Therapy of Laryngeal Cancer Cells.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Hongmei Zhu, Bo Yang, Yang Niu, Yongjiu Huang
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Abstract

Laryngeal cancer, a common malignant respiratory tumor, is primarily treated through surgery. However, challenges such as recurrence, metastasis, and drug resistance persist. In recent years, multifunctional drug delivery systems (DDS) based on nanoparticles have shown great potential in improving drug loading and release. We developed a biocompatible core-shell nanoparticle system with a zinc-based metal-organic framework (MOF) as the core, named CP1. The shell, composed of polyethyleneimine (PEI), folic acid, and calcium carbonate, forms a composite called CaCO3-PEI-FA. This system enhances biocompatibility and increases the efficacy of biomedical applications. Encapsulating CP1 within the CaCO3-PEI-FA shell allows for the targeted delivery of the anticancer drug doxorubicin (DOX) to laryngeal cancer cells (Hep-2), resulting in the CaCO3-PEI-FA@CP1@DOX system. The CaCO3-PEI-FA composite exhibits strong fluorescence with a peak around 350 nm, confirming successful synthesis and demonstrating its potential as a bioimaging probe. Importantly, the nanoparticle system without DOX showed low toxicity to normal human skin fibroblasts (HSF). In vitro cytology experiments revealed a 38% inhibition rate of Hep-2 cells after 24 h, highlighting the nanocomposite's significant potential in inhibiting laryngeal cancer cell proliferation and inducing apoptosis, underscoring its promise in targeted laryngeal cancer therapy.

用于喉癌细胞靶向治疗的荧光 MOF-Core CaCO₃-PEI-FA 外壳纳米粒子的设计与体外评估
喉癌是一种常见的呼吸道恶性肿瘤,主要通过手术治疗。然而,复发、转移和耐药性等挑战依然存在。近年来,基于纳米颗粒的多功能给药系统(DDS)在改善药物负载和释放方面显示出巨大潜力。我们开发了一种以锌基金属有机框架(MOF)为核心的生物相容性核壳纳米粒子系统,命名为 CP1。外壳由聚乙烯亚胺(PEI)、叶酸和碳酸钙组成,形成一种名为 CaCO3-PEI-FA 的复合材料。该系统增强了生物相容性,提高了生物医学应用的功效。将 CP1 封装在 CaCO3-PEI-FA 外壳中,可向喉癌细胞(Hep-2)靶向输送抗癌药物多柔比星(DOX),从而形成 CaCO3-PEI-FA@CP1@DOX 系统。CaCO3-PEI-FA 复合物显示出强烈的荧光,峰值在 350 纳米左右,证实了合成的成功,并证明了其作为生物成像探针的潜力。重要的是,不含 DOX 的纳米粒子系统对正常人皮肤成纤维细胞(HSF)的毒性很低。体外细胞学实验显示,该纳米复合材料在 24 小时后对 Hep-2 细胞的抑制率达到了 38%,突出了其在抑制喉癌细胞增殖和诱导细胞凋亡方面的巨大潜力,彰显了其在喉癌靶向治疗方面的前景。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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