Osteoblast Response to Widely Ranged Texturing Conditions Obtained through High Power Laser Beams on Ti Surfaces.

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL
Federico Alessandro Ruffinatti, Tullio Genova, Ilaria Roato, Martina Perin, Giorgia Chinigò, Riccardo Pedraza, Olivio Della Bella, Francesca Motta, Elisa Aimo Boot, Domenico D'Angelo, Giorgio Gatti, Giorgia Scarpellino, Luca Munaron, Federico Mussano
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Abstract

Titanium and titanium alloys are the prevailing dental implant materials owing to their favorable mechanical properties and biocompatibility, but how roughness dictates the biological response is still a matter of debate. In this study, laser texturing was used to generate eight paradigmatic roughened surfaces, with the aim of studying the early biological response elicited on MC3T3-E1 pre-osteoblasts. Prior to cell tests, the samples underwent SEM analysis, optical profilometry, protein adsorption assay, and optical contact angle measurement with water and diiodomethane to determine surface free energy. While all the specimens proved to be biocompatible, supporting similar cell viability at 1, 2, and 3 days, surface roughness could impact significantly on cell adhesion. Factorial analysis and linear regression showed, in a robust and unprecedented way, that an isotropic distribution of deep and closely spaced valleys provides the best condition for cell adhesion, to which both protein adsorption and surface free energy were highly correlated. Overall, here the authors provide, for the first time, a thorough investigation of the relationship between roughness parameters and osteoblast adhesion that may be applied to design and produce new tailored interfaces for implant materials.

成骨细胞对通过高功率激光束在钛表面获得的各种纹理条件的反应
钛和钛合金因其良好的机械性能和生物相容性而成为最常用的牙科植入材料,但粗糙度如何决定生物反应仍是一个争论不休的问题。本研究使用激光纹理技术生成了八个典型的粗糙表面,目的是研究 MC3T3-E1 前成骨细胞的早期生物反应。在进行细胞测试之前,对样品进行了扫描电镜分析、光学轮廓测定、蛋白质吸附测定以及水和二碘甲烷的光学接触角测量,以确定表面自由能。虽然所有试样都被证明具有生物相容性,在 1、2 和 3 天内支持相似的细胞存活率,但表面粗糙度会对细胞粘附性产生显著影响。因子分析和线性回归以前所未有的稳健方式表明,各向同性的深谷分布和紧密间隔为细胞粘附提供了最佳条件,蛋白质吸附和表面自由能与之高度相关。总之,作者首次对粗糙度参数与成骨细胞粘附性之间的关系进行了深入研究,并将其应用于设计和生产新的植入材料定制界面。
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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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