In Vivo Biocompatibility of Synechococcus sp. PCC 7002-Integrated Scaffolds for Skin Regeneration.

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL
Benedikt Fuchs, Sinan Mert, Constanze Kuhlmann, Alexandra Birt, Daniel Hofmann, Paul Severin Wiggenhauser, Riccardo E Giunta, Myra N Chavez, Jörg Nickelsen, Thilo Ludwig Schenck, Nicholas Moellhoff
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Abstract

Cyanobacteria, commonly known as blue-green algae, are prevalent in freshwater systems and have gained interest for their potential in medical applications, particularly in skin regeneration. Among these, Synechococcus sp. strain PCC 7002 stands out because of its rapid proliferation and capacity to be genetically modified to produce growth factors. This study investigates the safety of Synechococcus sp. PCC 7002 when used in scaffolds for skin regeneration, focusing on systemic inflammatory responses in a murine model. We evaluated the following three groups: scaffolds colonized with genetically engineered bacteria producing hyaluronic acid, scaffolds with wild-type bacteria, and control scaffolds without bacteria. After seven days, we assessed systemic inflammation by measuring changes in cytokine profiles and lymphatic organ sizes. The results showed no significant differences in spleen, thymus, and lymph node weights, indicating a lack of overt systemic toxicity. Blood cytokine analysis revealed elevated levels of IL-6 and IL-1β in scaffolds with bacteria, suggesting a systemic inflammatory response, while TNF-α levels remained unaffected. Proteome profiling identified distinct cytokine patterns associated with bacterial colonization, including elevated inflammatory proteins and products, indicative of acute inflammation. Conversely, control scaffolds exhibited protein profiles suggestive of a rejection response, characterized by increased levels of cytokines involved in T and B cell activation. Our findings suggest that Synechococcus sp. PCC 7002 does not appear to cause significant systemic toxicity, supporting its potential use in biomedical applications. Further research is necessary to explore the long-term effects and clinical implications of these responses.

用于皮肤再生的 Synechococcus sp.
蓝藻,俗称蓝绿藻,在淡水系统中十分普遍,因其在医疗应用,特别是皮肤再生方面的潜力而备受关注。其中,Synechococcus sp. 菌株 PCC 7002 因其快速增殖和可通过基因改造产生生长因子而脱颖而出。本研究调查了 Synechococcus sp. PCC 7002 用于皮肤再生支架时的安全性,重点是小鼠模型中的全身炎症反应。我们对以下三组进行了评估:带有产生透明质酸的基因工程细菌的支架、带有野生型细菌的支架以及不含细菌的对照支架。七天后,我们通过测量细胞因子谱和淋巴器官大小的变化来评估全身炎症。结果显示,脾脏、胸腺和淋巴结重量没有明显差异,表明没有明显的全身毒性。血液细胞因子分析表明,带有细菌的支架中 IL-6 和 IL-1β 水平升高,表明存在全身炎症反应,而 TNF-α 水平未受影响。蛋白质组分析确定了与细菌定植相关的独特细胞因子模式,包括炎症蛋白和产物的升高,表明存在急性炎症。与此相反,对照支架的蛋白质图谱显示出排斥反应,其特点是参与 T 细胞和 B 细胞活化的细胞因子水平升高。我们的研究结果表明,Synechococcus sp. PCC 7002 似乎不会引起明显的全身毒性,这支持了其在生物医学应用中的潜在用途。要探索这些反应的长期影响和临床意义,还需要进一步的研究。
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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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