A pH-Responsive Ti-Based Local Drug Delivery System for Osteosarcoma Therapy.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL

Journal of Functional Biomaterials Pub Date : 2024-10-21 DOI:10.3390/jfb15100312

Qinle Xiao, Changjun Wan, Zhe Zhang, Hui Liu, Pingting Liu, Qianli Huang, Dapeng Zhao

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引用次数: 0

Abstract

Osteosarcoma is one of the major bone cancers, especially for youngsters. The current treatment usually requires systemic chemotherapy and the removal of bone tumors. Titanium (Ti)-based implants can be modified as local drug delivery (LDD) systems for controllable and localized chemotherapeutic drug release. In this work, a pH-responsive Ti-based LDD prototype was designed by introducing polydopamine (PDA) to release doxorubicin (DOX) around osteosarcoma cells with low pH. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and a contact angle meter were applied for surface characterization. Both direct and indirect cell culture modes were performed for biocompatibility and biofunction assessments. The results indicate that the Ti-based LDD prototype exhibits significant pH-dependent DOX release. The cumulative release can reach up to approximately 40% at pH = 6.0 after 72 h, but only around 20% at pH = 7.4. The Ti-based LDD implant shows good biocompatibility with approximately 93% viability of MC3T3 cells after direct culture in vitro for 24 h. Both direct and indirect culture modes verify the good anti-osteosarcoma function of the LDD implant, which should be attributed to the pH-responsive release of DOX.

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用于骨肉瘤治疗的 pH 值响应型钛基局部给药系统

骨肉瘤是主要骨癌之一，尤其是对青少年而言。目前的治疗通常需要全身化疗和切除骨肿瘤。钛（Ti）基植入物可改性为局部给药系统（LDD），实现可控的局部化疗药物释放。本研究通过引入聚多巴胺（PDA）设计了一种 pH 响应型钛基 LDD 原型，可在低 pH 值的骨肉瘤细胞周围释放多柔比星（DOX）。傅立叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和接触角仪被用于表面表征。在生物相容性和生物功能评估方面，采用了直接和间接细胞培养模式。结果表明，基于钛的 LDD 原型表现出显著的 DOX 释放依赖性（pH 值）。在 pH = 6.0 的条件下，72 小时后的累积释放量可达约 40%，但在 pH = 7.4 的条件下，释放量仅为 20%左右。钛基 LDD 植入物显示出良好的生物相容性，体外直接培养 24 小时后，MC3T3 细胞的存活率约为 93%。直接和间接培养模式都验证了 LDD 植入物良好的抗骨肉瘤功能，这应归功于 DOX 的 pH 响应释放。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.