Caffeic acid inhibits Staphylococcus aureus-induced endometritis through regulating AMPKα/mTOR/HIF-1α signalling pathway

IF 5.3
Lu Cao, Junbao Liu, Cong Ye, Yubo Hu, Rui Qin
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引用次数: 0

Abstract

Endometritis is mostly caused by childbirth or postpartum uterine infection. It is one of the important reasons leading to female infertility. Caffeic acid (CA) and its derivatives are widely found in some foods and traditional Chinese medicine, and have biological activities such as antioxidant, free radical scavenging, anti-inflammatory, and anti-infection. In this study, we aimed to explore the effect of CA on Staphylococcus aureus-induced endometritis. The contents of TNF-α and IL-1β were detected by ELISA in S. aureus-induced endometritis model. Western blot assay was used to detect the expression of AMPKα/mTOR/HIF-1α pathway related proteins and GPX4 expression. In addition, the concentrations of MDA, GSH, and iron were tested by the assay kits. Compared with the model group, CA treatment significantly alleviated S. aureus-induced uterine injury, MPO activity, the contents of inflammatory factors TNF-α and IL-1β, and NF-κB activation. Meanwhile, CA significantly inhibited S. aureus-induced ferroptosis, as confirmed by decreased MDA and iron concentration and up-regulated GPX4 expression and GSH level. Furthermore, CA attenuated S. aureus-induced HIF-1α and phosphorylated mTOR expression and increased phosphorylated AMPK expression. In conclusion, CA inhibits inflammation and ferroptosis by regulating AMPKα/mTOR/HIF-1α signalling pathway to alleviate S. aureus-induced endometritis in mice.

Abstract Image

咖啡酸通过调节AMPKα/mTOR/HIF-1α信号通路抑制金黄色葡萄球菌诱发的子宫内膜炎
子宫内膜炎多由分娩或产后子宫感染引起。它是导致女性不孕的重要原因之一。咖啡酸(CA)及其衍生物广泛存在于一些食品和中药中,具有抗氧化、清除自由基、抗炎、抗感染等生物活性。本研究旨在探讨CA对金黄色葡萄球菌引起的子宫内膜炎的影响。在金黄色葡萄球菌诱导的子宫内膜炎模型中,用酶联免疫吸附法检测 TNF-α 和 IL-1β 的含量。用 Western 印迹法检测 AMPKα/mTOR/HIF-1α 通路相关蛋白的表达和 GPX4 的表达。此外,检测试剂盒还检测了 MDA、GSH 和铁的浓度。与模型组相比,CA能明显减轻金黄色葡萄球菌诱导的子宫损伤、MPO活性、炎症因子TNF-α和IL-1β的含量以及NF-κB的活化。同时,CA 能明显抑制金黄色葡萄球菌诱导的铁变态反应,其表现为降低 MDA 和铁浓度,上调 GPX4 表达和 GSH 水平。此外,CA 还抑制了金黄色葡萄球菌诱导的 HIF-1α 和磷酸化 mTOR 的表达,并增加了磷酸化 AMPK 的表达。总之,CA通过调节AMPKα/mTOR/HIF-1α信号通路来抑制炎症和铁变态反应,从而缓解金黄色葡萄球菌诱导的小鼠子宫内膜炎。
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来源期刊
CiteScore
11.50
自引率
0.00%
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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