Impact of Biofilms on Surface Properties of Polymethyl Methacrylate (PMMA) Resins.

IF 3.5 4区 生物学 Q2 MICROBIOLOGY
Syed Ali Danish Kazmi, Tahira Soomro, Rimsha Soomro, Fouzia Zeeshan Khan, Bushra Jabeen, Tanveer Abbas, Yasir Raza, Zulfiqar Ali Mirani
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引用次数: 0

Abstract

Poly(methyl methacrylate) (PMMA) resins are widely used in medical and dental applications. Their susceptibility to bacterial biofilm formation poses significant challenges related to material degradation and infection risk. This study investigated the effects of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) biofilms on PMMA resin surface properties over a 45-day period at 35°C. The study examined various parameters including biofilm adhesion, morphology, surface roughness, hydrophobicity, solid fraction, and zeta potential. PMMA resin specimens were inoculated with bacteria and incubated for 45 days. Biofilm adhesion was visually assessed, while surface characterization was conducted using scanning electron microscopy (SEM), atomic force microscopy (AFM), roughness analysis, contact angle measurements, solid fraction determination, and zeta potential analysis. The P. aeruginosa and S. aureus isolates were selected based on their biofilm-positive characteristics, which were further confirmed using Congo red and biofilm formation assays through crystal violet staining and spectrophotometric analysis. The results demonstrated robust biofilm adhesion on PMMA surfaces. SEM and AFM imaging revealed textured surfaces with elevated structures and depressions within the biofilm matrix. Biofilm-exposed resins exhibited significantly increased roughness (Ra = 164.5 nm, Rq = 169.5 nm) and hydrophobicity (mean angle = 85.5°-90.5°) compared to control samples (Ra = 38-50 nm, angle = 55°). Solid fraction measurements indicated a denser biofilm matrix on exposed resins (0.908) compared to controls (0.65). Additionally, zeta potential values were more negative for biofilm-exposed resins (mean = -84.2 mV) than controls (-45.0 mV). These findings underscore the substantial alterations in PMMA resin surface properties induced by bacterial biofilms, emphasizing the critical need for strategies to prevent biofilm formation and mitigate associated risks in healthcare settings. Future research should focus on developing anti-biofilm coatings or treatments to preserve the integrity and functionality of PMMA materials.

生物膜对聚甲基丙烯酸甲酯 (PMMA) 树脂表面特性的影响。
聚甲基丙烯酸甲酯(PMMA)树脂广泛应用于医疗和牙科领域。它们容易形成细菌生物膜,这给材料降解和感染风险带来了巨大挑战。本研究调查了铜绿假单胞菌(P. aeruginosa)和金黄色葡萄球菌(S. aureus)生物膜在 35°C 下 45 天内对 PMMA 树脂表面特性的影响。该研究考察了各种参数,包括生物膜的附着力、形态、表面粗糙度、疏水性、固体分数和 zeta 电位。在 PMMA 树脂试样中接种细菌并培养 45 天。对生物膜的附着力进行目测评估,同时使用扫描电子显微镜(SEM)、原子力显微镜(AFM)、粗糙度分析、接触角测量、固体分数测定和 zeta 电位分析进行表面表征。根据铜绿假单胞菌和金黄色葡萄球菌的生物膜阳性特征选择了这两种分离物,并使用刚果红和生物膜形成测定法通过水晶紫染色和分光光度分析进一步确认了这些特征。结果表明,PMMA 表面的生物膜附着力很强。扫描电子显微镜和原子力显微镜成像显示了生物膜基质内具有隆起结构和凹陷的纹理表面。与对照样品(Ra = 38-50 nm,角度 = 55°)相比,暴露于生物膜的树脂表现出明显增加的粗糙度(Ra = 164.5 nm,Rq = 169.5 nm)和疏水性(平均角度 = 85.5°-90.5°)。固体组分测量结果表明,与对照组(0.65)相比,暴露树脂上的生物膜基质密度更高(0.908)。此外,生物膜暴露树脂的 zeta 电位值(平均 = -84.2 mV)比对照组(-45.0 mV)更负。这些发现强调了细菌生物膜对 PMMA 树脂表面特性的重大改变,强调了在医疗保健环境中防止生物膜形成和降低相关风险的策略的迫切需要。未来的研究应侧重于开发抗生物膜涂层或处理方法,以保持 PMMA 材料的完整性和功能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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