{"title":"Handgrip-Ring Structure Sensing Probe Assisted Multiple Signal Amplification Strategy for Sensitive and Label-Free Single-Stranded Nucleic Acid Analysis.","authors":"Ying Ren, Yu He, Ping Li","doi":"10.1155/2024/6832856","DOIUrl":null,"url":null,"abstract":"<p><p>Precise and efficient identification of single-stranded nucleic acids is crucial for both pathological research and early diagnosis of diseases, such as cancers. Therefore, we have devised a novel biosensor that utilizes an elegantly designed handgrip-ring structure sensing probe to enhance the detection sensitivity and reduce background signals. The handgrip-ring structure sensing probe combines ring padlock-based target recognition and hairpin structure probe-based signal amplification. The target sequences form a binding interaction with the ring padlock in the sensing probe, leading to the elongation of the single-stranded chain with the assistance of polymerase. This elongation step results in the release of the hairpin probe, triggering a signal amplification process. This design significantly minimized the potential discrepancies that may occur during the signal amplification process, hence bestowing the approach with a low level of background signals. By utilizing this innovative design, the current biosensor demonstrates a remarkable ability to detect miRNA with a limit as low as 376 aM and single-stranded DNA sequences with a limit as low as 45.3 aM. In addition, it possesses exceptional discrimination capabilities. The efficacy of this approach in diagnosing targets was also effectively proved by the rational redesign of the ring padlock.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2024 ","pages":"6832856"},"PeriodicalIF":2.3000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511596/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Methods in Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1155/2024/6832856","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Precise and efficient identification of single-stranded nucleic acids is crucial for both pathological research and early diagnosis of diseases, such as cancers. Therefore, we have devised a novel biosensor that utilizes an elegantly designed handgrip-ring structure sensing probe to enhance the detection sensitivity and reduce background signals. The handgrip-ring structure sensing probe combines ring padlock-based target recognition and hairpin structure probe-based signal amplification. The target sequences form a binding interaction with the ring padlock in the sensing probe, leading to the elongation of the single-stranded chain with the assistance of polymerase. This elongation step results in the release of the hairpin probe, triggering a signal amplification process. This design significantly minimized the potential discrepancies that may occur during the signal amplification process, hence bestowing the approach with a low level of background signals. By utilizing this innovative design, the current biosensor demonstrates a remarkable ability to detect miRNA with a limit as low as 376 aM and single-stranded DNA sequences with a limit as low as 45.3 aM. In addition, it possesses exceptional discrimination capabilities. The efficacy of this approach in diagnosing targets was also effectively proved by the rational redesign of the ring padlock.
精确高效地识别单链核酸对于病理研究和癌症等疾病的早期诊断至关重要。因此,我们设计了一种新型生物传感器,利用设计优雅的手握环结构传感探针来提高检测灵敏度并减少背景信号。手握环结构传感探针结合了基于挂锁环的目标识别和基于发夹结构探针的信号放大。目标序列与传感探针中的环状挂锁形成结合作用,在聚合酶的协助下导致单链的延伸。这一延伸步骤导致发夹探针的释放,引发信号放大过程。这种设计大大减少了信号放大过程中可能出现的偏差,因此这种方法的背景信号水平很低。通过采用这种创新设计,目前的生物传感器显示出卓越的能力,可检测低至 376 aM 的 miRNA 和低至 45.3 aM 的单链 DNA 序列。此外,它还具有卓越的分辨能力。对环形挂锁的合理重新设计也有效证明了这种方法在诊断目标方面的功效。
期刊介绍:
Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical.
Subject areas include (but are by no means limited to):
Separation
Spectroscopy
Mass spectrometry
Chromatography
Analytical Sample Preparation
Electrochemical analysis
Hyphenated techniques
Data processing
As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.