An Outbreak of Acute Hemorrhagic Conjunctivitis Caused by Coxsackievirus A24 in Eastern Uttar Pradesh, India 2023.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2024-01-01 Epub Date: 2024-10-22 DOI:10.1159/000540952

Sthita Pragnya Behera, Nalini Mishra, Ramyash Yadav, Aishwarya Shukla, Moni Kumari, Sonal Rajput, Imbisat Fatma, Ashutosh Tiwari, Prashansha Srivastava, Shashikant Tiwari, Rajeev Singh, Satish S Ranawade, Manoj Murhekar, Gaurav Raj Dwivedi

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引用次数: 0

Abstract

Introduction: Acute hemorrhagic conjunctivitis (AHC) outbreaks are caused mostly by viruses. During July-August 2023, there was a sudden spike in acute hemorrhage conjunctivitis cases in Eastern Uttar Pradesh, India. To identify the etiological and gain molecular epidemiology of the agent, the study was conducted.

Methodology: Conjunctival swabs were collected from patients (n = 128) with presumed acute hemorrhage conjunctivitis visiting two tertiary care hospitals.

Results: Enteroviruses infection was identified in 96 (75%) patients. In these patients, coxsackievirus A24 (CV-A24) infection was further confirmed by targeting the genetic regions of 3C protease and VP1. Furthermore, the study established the outbreak was caused by the genotype IV of CV-A24 with the highest genetic similarity with CV-A24 reported from Northeast India, China, and Pakistan circulating during the same period. The comparison of our study sequences with earlier Indian outbreak strains (2007) revealed four amino acid substitutions at the 3C region ("S21N," "V30I," "S66I," and "V75I") and three non-synonymous mutations at the VP1 region ("L16I," "P21S," and "N301D").

Conclusion: The study findings revealed that the AHC outbreak was caused by genotype IV of CV-A24 in this region. Molecular identification accompanied by phylogenetic analysis will be useful in studying the enterovirus epidemiology associated with AHC outbreaks.

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2023 年印度北方邦东部爆发柯萨奇病毒 A24 引起的急性出血性结膜炎。

导言：急性出血性结膜炎（AHC）的爆发主要是由病毒引起的。2023 年 7 月至 8 月期间，印度北方邦东部的急性出血性结膜炎病例突然激增。为了确定病原体并获得分子流行病学，我们开展了这项研究：方法：从两家三级医院就诊的推测患有急性出血性结膜炎的患者（n = 128）中采集结膜拭子：结果：96 名（75%）患者确定感染了肠道病毒。结果：在 96 名（75%）患者中发现了肠道病毒感染，其中柯萨奇病毒 A24（CV-A24）感染是通过靶向 3C 蛋白酶和 VP1 基因区进一步确认的。此外，研究还确定疫情是由 CV-A24 基因型 IV 引起的，该基因型与同期在印度东北部、中国和巴基斯坦流行的 CV-A24 基因型相似度最高。将我们的研究序列与早期印度疫情株（2007 年）进行比较，发现 3C 区有四个氨基酸置换（"S21N"、"V30I"、"S66I "和 "V75I"），VP1 区有三个非同义突变（"L16I"、"P21S "和 "N301D"）：研究结果表明，该地区爆发的 AHC 是由 CV-A24 基因型 IV 引起的。分子鉴定和系统发育分析将有助于研究与 AHC 爆发相关的肠道病毒流行病学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.