Myrtenol's Effectiveness against Hospital-Acquired Methicillin-Resistant Staphylococcus aureus: Targeting Antibiofilm and Antivirulence Properties.

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI:10.1155/2024/8832448
Amal F Makled, Azza Z Labeeb, Eman A E Badr, Amany M Abdelmaksoud, Safa R Elfiky, Asmaa K Amer, Asmaa S Sleem
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引用次数: 0

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) several years ago highlighted the challenge of multidrug-resistant infections, emphasizing the critical need for innovative treatment approaches. Myrtenol, known for its antibacterial and antibiofilm properties, holds promise as a potential treatment option. This study aimed to evaluate the effectiveness of myrtenol against MRSA. The collected MRSA isolates were assessed for antimicrobial susceptibility following the Clinical and Laboratory Standards Institute (CLSI) guidelines 2023. Biofilm formation by MRSA was evaluated using the tissue culture plate (TCP) technique. The minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and minimal biofilm inhibitory concentration (MBIC) of myrtenol against MRSA were determined both individually and in combination with antibiotics. Real-time PCR was employed to investigate the impact of myrtenol on the expression of virulence genes (sarA, agrA, and icaD) across the isolates. In this study, MRSA was identified in 90 out of 400 cases (22.5%) of hospital-acquired pathogens. Among the collected MRSA isolates, 53 out of 90 (59%) were found to produce biofilms. The MIC of myrtenol was comparable to the MBC across all tested isolates, they were almost the same. Combinations of myrtenol with most tested antibiotics exhibited synergistic effects exceeding 60%. Among the 53 biofilm-producing isolates, 45 isolates (85%) expressed the sarA gene, 49% expressed the agrA gene, and all biofilm-producing MRSA isolates (100%) expressed the icaD gene. A notable reduction in the relative quantity (RQ) values of virulence gene expression was observed after treatment with the MBIC of myrtenol across all tested isolates. Myrtenol demonstrated strong antimicrobial activity against MRSA, notably reducing the expression of key virulence genes linked to biofilm formation. This suggests its potential as a therapeutic agent for treating biofilm-associated MRSA infections.

肉豆蔻醇对医院获得性耐甲氧西林金黄色葡萄球菌的有效性:针对抗生物膜和抗病毒特性。
几年前出现的耐甲氧西林金黄色葡萄球菌(MRSA)凸显了耐多药感染的挑战,强调了对创新治疗方法的迫切需要。以抗菌和抗生物膜特性著称的桃金娘醇有望成为一种潜在的治疗方法。本研究旨在评估肉毒酚对 MRSA 的疗效。根据临床和实验室标准协会(CLSI)2023 准则,对收集到的 MRSA 分离物进行了抗菌药敏感性评估。采用组织培养板(TCP)技术对 MRSA 的生物膜形成进行了评估。测定了肉毒碱单独或与抗生素联合使用时对 MRSA 的最小抑菌浓度 (MIC)、最小杀菌浓度 (MBC) 和最小生物膜抑菌浓度 (MBIC)。研究人员采用实时 PCR 技术研究了肉毒酚对不同分离株毒力基因(sarA、agrA 和 icaD)表达的影响。在这项研究中,400 例医院获得性病原体中有 90 例(22.5%)鉴定出 MRSA。在收集到的 MRSA 分离物中,发现 90 个中有 53 个(59%)产生了生物膜。在所有检测的分离物中,myrtenol 的 MIC 值与 MBC 值相当,几乎相同。桃金娘醇与大多数测试抗生素的组合协同效应超过了 60%。在 53 个产生生物膜的分离物中,45 个分离物(85%)表达 sarA 基因,49% 表达 agrA 基因,所有产生生物膜的 MRSA 分离物(100%)都表达 icaD 基因。所有接受测试的分离物在使用糠萜醇 MBIC 处理后,毒力基因表达的相对数量 (RQ) 值都明显降低。肉豆蔻醇对 MRSA 具有很强的抗菌活性,能显著降低与生物膜形成有关的关键毒力基因的表达。这表明它具有治疗与生物膜相关的 MRSA 感染的潜力。
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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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