A multidisciplinary approach to forensic biological profiling on a single tooth and nail sample.

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
Rogier van der Hulst, Reza R R Gerretsen, Lisette M Kootker, Sanne W L Palstra, Arnoud J Kal, Saskia T M Ammer, Shirley P Verschoor, Lennaert C P Borra, Carina S M van Leeuwen, Miranda Verschraagen, Gareth R Davies, Roelof-Jan Oostra, Daniël J Touw
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Abstract

Introduction: Analysis of a single tooth and nail can provide valuable forensic information, including year of birth, year of death, age, sex, DNA-profile, geographic residence during childhood and at time of death and drug exposure. The aim is to minimize the amount of used bodily material and to validate the applicability of a multidisciplinary sampling protocol.

Methods: A nail of the big toe, a tooth and blood of seven deceased individuals were collected postmortem. Collected materials were sampled and segmented in accordance with the multidisciplinary sampling protocol. DNA analysis was conducted on the pulp of the tooth, isotope analysis (Sr, Pb, O and C) on the enamel and 14C-, toxicological and tooth cementum annulation analysis on root segments. DNA-, isotope (Sr, Pb, O and C) -, toxicological-, and 14C -analysis were conducted on toenail segments. The acquired DNA profiles were compared with profiles acquired from blood.

Results: Material from seven deceased persons was analysed. 45 out of 56 analyses on dental samples were successful, constituting a success rate of 80%. Additionally, 27 out of 35 analyses were successful on nail samples, yielding a success rate of 77%. DNA-, toxicological and 14C- analyses performed better in nail than in tooth. Isotope analyses performed better in tooth than in nail. A profile with personal characteristics was constructed and matched for 62% of parameters with collected medical information.

Conclusion: The performed sampling protocol for simultaneous multidisciplinary forensic analysis on a single tooth and nail sample provided applicable results and valuable information.

对单一牙齿和指甲样本进行法医生物特征分析的多学科方法。
介绍:对一颗牙齿和指甲的分析可以提供宝贵的法医信息,包括出生年份、死亡年份、年龄、性别、DNA特征、童年和死亡时的居住地以及药物接触情况。其目的是尽量减少使用的身体材料数量,并验证多学科采样方案的适用性:方法:采集了七名死者的大脚趾指甲、牙齿和血液。根据多学科取样协议对收集的材料进行取样和分割。对牙髓进行了 DNA 分析,对牙釉质进行了同位素分析(Sr、Pb、O 和 C),对牙根部分进行了 14C、毒理学和牙骨质环化分析。对脚趾甲片段进行了 DNA、同位素(锶、铅、氧和碳)、毒理学和 14C 分析。获得的 DNA 图谱与从血液中获得的图谱进行了比较:结果:对七名死者的材料进行了分析。在 56 份牙科样本中,45 份分析成功,成功率为 80%。此外,对指甲样本进行的 35 次分析中有 27 次成功,成功率为 77%。指甲样本的 DNA、毒理学和 14C 分析结果均优于牙齿样本。同位素分析在牙齿上的效果好于指甲。我们建立了个人特征档案,其中 62% 的参数与收集到的医疗信息相匹配:对单一牙齿和指甲样本进行多学科同步法医分析的取样方案提供了适用的结果和有价值的信息。
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来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
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