Whole-genome comparative analysis of the genetic, virulence and antimicrobial resistance diversity of Campylobacter spp. from Spain

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
{"title":"Whole-genome comparative analysis of the genetic, virulence and antimicrobial resistance diversity of Campylobacter spp. from Spain","authors":"","doi":"10.1016/j.ijfoodmicro.2024.110940","DOIUrl":null,"url":null,"abstract":"<div><div>Whole-Genome Sequencing has the potential to be an effective method for surveillance of foodborne diseases. This study aims to determine the genetic relatedness and prevalence of virulence-associated genes and antimicrobial resistance determinants in 135 <em>Campylobacter jejuni</em>, seven <em>Campylobacter coli</em> and three <em>Campylobacter lari</em> isolates from the poultry supply chain and a hospital in Spain. The isolates showed a wide genetic diversity between and within species with Clonal Complex 21 the most frequent lineage found. Among species, <em>C. jejuni</em> showed the highest prevalence of virulence genes (287/333) in which a high occurring variability was observed in the capsule biosynthesis and transport, O-linked flagellar glycosylation and lipooligosaccharide biosynthesis loci, with a great impact of phase-variation that led to 72 different virulence gene patterns among all isolates. High prevalence (&gt; 90 %) of <em>bla</em><sub><em>OXA</em></sub>-type β-lactamase genes and mutations in DNA gyrase gene associated with fluoroquinolones resistance were observed, and at a frequency similar to the <em>tet(O)</em> gene in <em>C. jejuni</em> (93 %) and <em>C. coli</em> (86 %), both of which also harboured resistance determinants to aminoglycosides with a higher occurrence rate in <em>C. coli</em> (43 %), that was the only species in which mutations in the 23S ribosomal subunit conferring resistance to erythromycin were identified (43 %). The present study constitutes the largest genomic survey of <em>Campylobacter</em> isolates in Spain providing insight into the prevalence and diversity of the pathogen along the poultry supply chain in the country.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":null,"pages":null},"PeriodicalIF":5.0000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168160524003842","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Whole-Genome Sequencing has the potential to be an effective method for surveillance of foodborne diseases. This study aims to determine the genetic relatedness and prevalence of virulence-associated genes and antimicrobial resistance determinants in 135 Campylobacter jejuni, seven Campylobacter coli and three Campylobacter lari isolates from the poultry supply chain and a hospital in Spain. The isolates showed a wide genetic diversity between and within species with Clonal Complex 21 the most frequent lineage found. Among species, C. jejuni showed the highest prevalence of virulence genes (287/333) in which a high occurring variability was observed in the capsule biosynthesis and transport, O-linked flagellar glycosylation and lipooligosaccharide biosynthesis loci, with a great impact of phase-variation that led to 72 different virulence gene patterns among all isolates. High prevalence (> 90 %) of blaOXA-type β-lactamase genes and mutations in DNA gyrase gene associated with fluoroquinolones resistance were observed, and at a frequency similar to the tet(O) gene in C. jejuni (93 %) and C. coli (86 %), both of which also harboured resistance determinants to aminoglycosides with a higher occurrence rate in C. coli (43 %), that was the only species in which mutations in the 23S ribosomal subunit conferring resistance to erythromycin were identified (43 %). The present study constitutes the largest genomic survey of Campylobacter isolates in Spain providing insight into the prevalence and diversity of the pathogen along the poultry supply chain in the country.
对西班牙弯曲杆菌属的遗传、毒力和抗菌药耐药性多样性的全基因组比较分析。
全基因组测序有可能成为监测食源性疾病的有效方法。本研究旨在确定 135 个空肠弯曲菌、7 个大肠弯曲菌和 3 个拉里弯曲菌分离物中毒力相关基因和抗菌药耐药性决定因子的遗传相关性和流行率,这些分离物来自西班牙的家禽供应链和一家医院。分离物在物种之间和物种内部显示出广泛的遗传多样性,其中克隆复合体 21 是最常见的菌系。在物种中,空肠杆菌的毒力基因(287/333)流行率最高,其中在胶囊生物合成和转运、O-链鞭毛糖基化和脂寡糖生物合成位点中观察到了较高的发生变异性,相变的巨大影响导致所有分离株中出现了 72 种不同的毒力基因模式。观察到与氟喹诺酮类耐药性相关的 blaOXA 型 β-内酰胺酶基因和 DNA 回旋酶基因突变的高流行率(> 90%),其频率与空肠杆菌(93%)和大肠杆菌(86%)中的 tet(O)基因相似。大肠杆菌是唯一发现 23S 核糖体亚基突变对红霉素产生耐药性的物种(43%)。本研究是西班牙对弯曲杆菌分离物进行的最大规模的基因组调查,有助于深入了解病原体在该国家禽供应链中的流行情况和多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信