Molecular Markers for Analyses of Genetic Diversity within the Anastrepha fraterculus Complex with Emphasis on Argentine Populations.

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Insects Pub Date : 2024-09-27 DOI:10.3390/insects15100748

Ludvik M Gomulski, María Teresa Vera, Silvia B Lanzavecchia, Riccardo Piccinno, Giulia Fiorenza, Daniel De Luca, Beatriz N Carrizo, Juan Pedro R Bouvet, Valeria A Viana, Carlos Cárceres, Walther Enkerlin, Anna R Malacrida, Giuliano Gasperi

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引用次数: 0

Abstract

The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: "Mexican", "Venezuelan", "Andean", "Peruvian", "Ecuadorian", and the three Brazilian morphotypes "Brazilian-1", "Brazilian-2", and "Brazilian-3". Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.

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用于分析 Anastrepha fraterculus 复合体遗传多样性的分子标记，重点是阿根廷种群。

南美果蝇 Anastrepha fraterculus（Wiedmann）的分布范围很广，从墨西哥北部到中美洲，一直延伸到南美洲，是野生和栽培水果的一种多食性害虫。它是一个复杂的隐蔽物种，目前由 8 个公认的形态型组成："墨西哥型"、"委内瑞拉型"、"安第斯型"、"秘鲁型"、"厄瓜多尔型 "以及三个巴西型 "巴西-1 型"、"巴西-2 型 "和 "巴西-3 型"。能够识别该复合体成员物种的分子标记对于实施有效的害虫控制措施（如昆虫不育技术）至关重要。本研究的目的是评估利用内部转录间隔序列 2（ITS2）来鉴别 A. fraterculus隐蔽种群的几个成员（墨西哥、秘鲁和巴西-1）和一个相关种 Anastrepha schultzi Blanchard。分析结果表明，所评估的形态类型之间存在明显的遗传差异，可以将它们区分为 A. fraterculus隐蔽种群，并为它们之间的遗传关系提供了新的见解。ITS2 标记为开发分类学密钥提供了基础，可用于区分 A. fraterculus隐蔽种群中的隐蔽类群。ITS2 也是研究较少的物种 A. schultzi 的一个重要标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insects Agricultural and Biological Sciences-Insect Science

CiteScore

5.10

自引率

10.00%

发文量

1013

审稿时长

21.77 days

期刊介绍： Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.