Whole-genome sequencing analyses and antibiotic resistance situation of 48 Helicobacter pylori strains isolated in Zhejiang, China.

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2024-10-23 DOI:10.1186/s13099-024-00656-2

Yunhui Fang, Shiman Jiang, Xinxin Zhou, Wangxiao Zhou, Xinrong Jiang, Lifeng Chen, Mengting Wang, Yunbo Chen, Lanjuan Li

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Abstract

Purpose: In the Zhejiang region, research on Helicobacter pylori is lacking. The purpose of this study was to assess the extent of antibiotic resistance in H. pylori in this region, explore alternative methods for predicting the resistance patterns of H. pylori, and investigate the colonization of native gastric mucosa by other clades of H. pylori in the structure population of this bacterium.

Methods: Strains were cultured under microaerobic conditions, and antimicrobial susceptibility testing (AST) was performed via agar dilution. Whole-genome sequencing (WGS) was performed via next-generation sequencing (NGS) technology. Epidemiological data including data from this study and reported articles from Zhejiang, China, were included. Further analyses based on AST, WGS, and epidemiological date include virulence genes, antibiotic resistance-related mutations, and phylogenetic trees based on 7 housekeeping genes and core-genome single nucleotide polymorphisms (SNPs).

Results: The bacterial isolates in this study presented higher antibiotic resistance rates than previously reported, especially against levofloxacin and clarithromycin. The point mutation A2147G in 23 S rRNA is specific to clarithromycin resistance. Mutations at position/s 87 and/or 91 of the gyrA gene amino acid sequence are highly consistent with levofloxacin resistance highly. The point mutations C1707T in 23 S rRNA and E463K in the gyrB gene have not been previously documented in China. All the bacterial isolates belong to Asian branches in the structure population. The resistance rate to clarithromycin of isolates from hosts born after January 1, 1977 is statistically higher than that of hosts born before 1977.

Conclusion: Eradication therapy based on AST results is urgently needed in Zhejiang. The point mutation A2147G in 23 S rRNA and point mutations in the gyrA gene at amino acid/s 87 and/or 91 are sufficient for predicting resistance to clarithromycin and levofloxacin, respectively. The isolate with the mutation E463K in the gyrB gene represents a significant contribution to the field. Mutations in 23 S rRNA may offer valuable insights into the dynamics of H. pylori transmission among hosts.

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中国浙江省分离的 48 株幽门螺杆菌的全基因组测序分析和抗生素耐药性情况。

目的：浙江地区缺乏对幽门螺杆菌的研究。本研究旨在评估该地区幽门螺杆菌的抗生素耐药程度，探索预测幽门螺杆菌耐药模式的其他方法，并调查该细菌结构种群中其他支系幽门螺杆菌在本地胃黏膜的定植情况：方法：在微需氧条件下培养菌株，并通过琼脂稀释法进行抗菌药敏感性测试（AST）。通过新一代测序（NGS）技术进行全基因组测序（WGS）。流行病学数据包括本研究的数据和中国浙江的报道文章。基于 AST、WGS 和流行病学数据的进一步分析包括毒力基因、抗生素耐药性相关突变以及基于 7 个看家基因和核心基因组单核苷酸多态性（SNPs）的系统发生树：结果：本研究中分离的细菌对抗生素的耐药率高于之前的报道，尤其是对左氧氟沙星和克拉霉素。23 S rRNA 中的点突变 A2147G 是克拉霉素耐药性的特异性突变。gyrA基因氨基酸序列第87和/或91位的突变与左氧氟沙星抗性高度一致。23 S rRNA 中的点突变 C1707T 和 gyrB 基因中的点突变 E463K 以前在中国没有记录。所有细菌分离株在结构上均属于亚洲分支。据统计，1977 年 1 月 1 日之后出生的宿主分离的细菌对克拉霉素的耐药率高于 1977 年之前出生的宿主：结论：浙江地区亟需基于 AST 结果的根除治疗。23 S rRNA 中的点突变 A2147G 和 gyrA 基因中第 87 和/或 91 位氨基酸的点突变分别足以预测对克拉霉素和左氧氟沙星的耐药性。gyrB基因突变为E463K的分离株是对该领域的重大贡献。23 S rRNA的突变可能为了解幽门螺杆菌在宿主间的传播动态提供了宝贵的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).