Shear-Thinning Extrudable Hydrogels Based on Star Polypeptides with Antimicrobial Properties.

IF 5 3区 化学 Q1 POLYMER SCIENCE
Gels Pub Date : 2024-10-11 DOI:10.3390/gels10100652
Dimitrios Skoulas, Muireann Fallon, Katelyn J Genoud, Fergal J O'Brien, Deirdre Fitzgerald Hughes, Andreas Heise
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Abstract

Hydrogels with low toxicity, antimicrobial potency and shear-thinning behavior are promising materials to combat the modern challenges of increased infections. Here, we report on 8-arm star block copolypeptides based on poly(L-lysine), poly(L-tyrosine) and poly(S-benzyl-L-cysteine) blocks. Three star block copolypeptides were synthesized with poly(S-benzyl-L-cysteine) always forming the outer block. The inner block comprised either two individual blocks of poly(L-lysine) and poly(L-tyrosine) or a statistical block copolypeptide from both amino acids. The star block copolypeptides were synthesized by the Ring Opening Polymerization (ROP) of the protected amino acid N-carboxyanhydrides (NCAs), keeping the overall ratio of monomers constant. All star block copolypeptides formed hydrogels and Scanning Electron Microscopy (SEM) confirmed a porous morphology. The investigation of their viscoelastic characteristics, water uptake and syringe extrudability revealed superior properties of the star polypeptide with a statistical inner block of L-lysine and L-tyrosine. Further testing of this sample confirmed no cytotoxicity and demonstrated antimicrobial activity of 1.5-log and 2.6-log reduction in colony-forming units, CFU/mL, against colony-forming reference laboratory strains of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively. The results underline the importance of controlling structural arrangements in polypeptides to optimize their physical and biological properties.

基于具有抗菌特性的星型多肽的剪切稀化可挤出水凝胶。
具有低毒性、抗菌效力和剪切稀化行为的水凝胶是应对感染增加这一现代挑战的有前途的材料。在此,我们报告了基于聚(L-赖氨酸)、聚(L-酪氨酸)和聚(S-苄基-L-半胱氨酸)嵌段的 8 臂星形嵌段共聚多肽。我们合成了三种星形嵌段共聚多肽,聚(S-苄基-L-半胱氨酸)始终构成外嵌段。内嵌段由聚(L-赖氨酸)和聚(L-酪氨酸)的两个独立嵌段或由这两种氨基酸组成的统计嵌段共聚多肽组成。星型嵌段共聚多肽是通过受保护氨基酸 N-羧基酸酐(NCA)的开环聚合(ROP)合成的,单体的总体比例保持不变。所有星型嵌段共聚多肽都形成了水凝胶,扫描电子显微镜(SEM)证实了其多孔形态。对其粘弹性特征、吸水性和注射器挤出性的研究表明,具有 L-赖氨酸和 L-酪氨酸统计内嵌段的星形多肽具有更优越的特性。对该样品的进一步测试证实其无细胞毒性,对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的菌落形成参考实验室菌株的抗菌活性分别降低了 1.5 和 2.6 个菌落形成单位(CFU/mL)。这些结果凸显了控制多肽结构排列以优化其物理和生物特性的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
期刊介绍: The journal Gels (ISSN 2310-2861) is an international, open access journal on physical (supramolecular) and chemical gel-based materials. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the maximum length of the papers, and full experimental details must be provided so that the results can be reproduced. Short communications, full research papers and review papers are accepted formats for the preparation of the manuscripts. Gels aims to serve as a reference journal with a focus on gel materials for researchers working in both academia and industry. Therefore, papers demonstrating practical applications of these materials are particularly welcome. Occasionally, invited contributions (i.e., original research and review articles) on emerging issues and high-tech applications of gels are published as special issues.
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