Contribution of haptoglobin phenotypic variation to the presence of hyperhomocysteinemia in type 2 diabetics with and without angiopathy.

IF 3.6 3区医学 Q2 NUTRITION & DIETETICS

European Journal of Clinical Nutrition Pub Date : 2024-10-26 DOI:10.1038/s41430-024-01524-7

Isabel Ferreira, Manuel Bicho, Ana Valente

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引用次数: 0

Abstract

Background/aim: The genetic polymorphism of haptoglobin (Hp) has been associated with several cardiovascular risk factors, but a possible relationship between Hp phenotypic variation and increased levels of homocysteine (Hcy) and cysteine (Cy) is still unknown. The objective of this study is to evaluate the relationship between the Hp polymorphism and hyperhomocysteinemia (HHcy) and hypercysteinemia (HCy) in type 2 diabetics (T2D) with and without angiopathy (AGP).

Methods: A case-control study was carried out on 293 adults: Group I (GI) - 75 subjects with T2D and AGP; Group II (GII) - 75 subjects with T2D without AGP; Group III (GIII) - 143 controls. Plasma levels of Hcy, Cy and vitamin B₆ were measured by high performance liquid chromatography (HPLC) and vitamins B₉ and B₁₂ determined by electrochemiluminescence (ECL). The Hp polymorphism was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and peroxidase staining. The results were analyzed in SPSS®, version 26.0 with a significance of 95%.

Results: Mean Hcy concentrations were significantly lower in carriers of the Hp2-2 phenotype (6.14 µM; p = 0.046) compared to the other genotypes. The presence of Hp2-1 is associated with an approximately 3.3 times greater probability of occurrence of HHcy (p = 0.015) and 3.7 times greater probability occurrence of HCy (p = 0.021) in T2D with AGP.

Conclusion: The presence of the Hp2-1 phenotype is associated with the predisposition of HHcy and HCy in individuals with T2D and AGP, possibly through a positive heterosis mechanism. Carriers of the Hp2-2 phenotype appear to have a greater activation of the transsulfuration pathway in the Hcy cycle and consequent protection for its accumulation.

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有血管病变和无血管病变的 2 型糖尿病患者中出现高同型半胱氨酸血症的血红蛋白表型变异的贡献。

背景/目的：血红蛋白（Hp）的遗传多态性与多种心血管风险因素有关，但 Hp 表型变异与同型半胱氨酸（Hcy）和半胱氨酸（Cy）水平升高之间的可能关系尚不清楚。本研究旨在评估伴有或不伴有血管病变（AGP）的 2 型糖尿病患者（T2D）中 Hp 多态性与高同型半胱氨酸血症（HHcy）和高半胱氨酸血症（HCy）之间的关系：对 293 名成年人进行了病例对照研究：第一组（GI）--75 名患有 T2D 并伴有 AGP 的受试者；第二组（GII）--75 名患有 T2D 但不伴有 AGP 的受试者；第三组（GIII）--143 名对照组。血浆中 Hcy、Cy 和维生素 B6 的含量通过高效液相色谱法（HPLC）进行测定，维生素 B9 和 B12 通过电化学发光法（ECL）进行测定。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和过氧化物酶染色鉴定了 Hp 的多态性。结果用 SPSS® 26.0 版进行分析，显著性为 95%：结果：与其他基因型相比，Hp2-2 表型携带者的平均 Hcy 浓度明显较低（6.14 µM；p = 0.046）。在患有 AGP 的 T2D 患者中，Hp2-1 的存在与 HHcy 发生概率高出约 3.3 倍（p = 0.015）和 HCy 发生概率高出约 3.7 倍（p = 0.021）有关：结论：Hp2-1表型的存在与T2D和AGP患者易患HHcy和HCy有关，这可能是通过正异质性机制实现的。Hp2-2 表型的携带者在 Hcy 循环中的转硫化途径中似乎有更大的激活作用，从而保护了 Hcy 的积累。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Clinical Nutrition 医学-营养学

CiteScore

10.60

自引率

2.10%

发文量

189

审稿时长

3-6 weeks

期刊介绍： The European Journal of Clinical Nutrition (EJCN) is an international, peer-reviewed journal covering all aspects of human and clinical nutrition. The journal welcomes original research, reviews, case reports and brief communications based on clinical, metabolic and epidemiological studies that describe methodologies, mechanisms, associations and benefits of nutritional interventions for clinical disease and health promotion. Topics of interest include but are not limited to: Nutrition and Health (including climate and ecological aspects) Metabolism & Metabolomics Genomics and personalized strategies in nutrition Nutrition during the early life cycle Health issues and nutrition in the elderly Phenotyping in clinical nutrition Nutrition in acute and chronic diseases The double burden of ''malnutrition'': Under-nutrition and Obesity Prevention of Non Communicable Diseases (NCD)