Evaluation of nonpolar lipid extract antigen-based enzyme-linked immunosorbent assay for the serodiagnosis of tuberculosis

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-13 DOI:10.1016/j.diagmicrobio.2024.116560

Luana E. Araújo , Jéssica Petrilli , Carlos Oliveira , Thainá Horta , Paulo Estevão , Fabiana Rabe Carvalho , Claudete A. Araújo Cardoso , Thiago Marconi Cardoso , Luanna de Ângelis , Lilian Montenegro , Fred Luciano Neves Santos , Sérgio Arruda , Adriano Queiroz

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Abstract

This study assessed the diagnostic potential of nonpolar lipid extracts in enzyme-linked immunosorbent assays (ELISAs) for tuberculosis (TB) serodiagnosis. Nonpolar lipid extracts were harvested from Mycobacterium tuberculosis (Mtb) knockout in mce1 operon (∆mce1) and its parental wild type (WT) strains. IgM and IgG anti-nonpolar lipid serum levels were measured in TB patients (n=45), healthy individuals with positive (n=22) and negative (n=44) interferon-gamma release assay (IGRA) results, and symptomatic respiratory (SR) patients with negative TB tests (n=9). IgG anti-WT lipid distinguished TB patients from IGRA-positive individuals with 60% sensitivity and 77.3% specificity. Conversely, IgG anti-∆mce lipid levels didn't vary significantly across groups. Interestingly, most SR patients exhibited significantly higher IgM and IgG anti-WT lipid titers than the IGRA-positive and -nega groups. While the overall diagnostic potential of Mtb nonpolar lipids was limited, the impaired immunogenecity of Δmce1 lipid extract suggests that some missing lipid classes in this extract can potentially induce antibody production in TB patients.

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评估基于非极性脂质提取物抗原的酶联免疫吸附试验在结核病血清诊断中的应用。

本研究评估了非极性脂质提取物在用于结核病（TB）血清诊断的酶联免疫吸附试验（ELISA）中的诊断潜力。非极性脂质提取物取自 mce1 操作子敲除的结核分枝杆菌（Mtb）（Δmce1）及其亲本野生型（WT）菌株。对结核病患者（45 人）、干扰素-γ 释放测定（IGRA）结果为阳性（22 人）和阴性（44 人）的健康人以及结核病检测结果为阴性的有症状呼吸道（SR）患者（9 人）的血清 IgM 和 IgG 抗非极性脂质水平进行了测定。IgG 抗 WT 脂质可将肺结核患者与 IGRA 阳性者区分开来，灵敏度为 60%，特异性为 77.3%。相反，IgG 抗Δmce 脂质水平在各组间没有显著差异。有趣的是，大多数 SR 患者的 IgM 和 IgG 抗 WT 脂质滴度明显高于 IGRA 阳性组和-nega 组。虽然Mtb非极性脂质的总体诊断潜力有限，但Δmce1脂质提取物的免疫原性受损表明，该提取物中某些缺失的脂质类别有可能诱导肺结核患者产生抗体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.