The effect of human umbilical cord mesenchymal stem cells combined with concentrated growth factor on repairing necrotic pulp caused by dental caries.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-26 DOI:10.4012/dmj.2024-007

Feifei Wang, Jie Wang, Lijie Zhang, Shifeng Fan, Siyu Liu

{"title":"The effect of human umbilical cord mesenchymal stem cells combined with concentrated growth factor on repairing necrotic pulp caused by dental caries.","authors":"Feifei Wang, Jie Wang, Lijie Zhang, Shifeng Fan, Siyu Liu","doi":"10.4012/dmj.2024-007","DOIUrl":null,"url":null,"abstract":"This study investigated the impact of combining human umbilical cord mesenchymal stem cells (hUC-MSCs) with concentrated growth factor (CGF) on regenerating necrotic pulp. Ten-month-old male Bama miniature pigs were divided into control and caries groups. The experimental teeth were randomly divided into three groups: caries untreated, Ca(OH)2, and engineering dental pulp-like tissue (EDPT). hUC-MSCs and CGF scaffold were combined to construct EDPT, and the histological structure was observed. Odontoblasts and dental pulp cells were counted in each group. The results showed that hUC-MSCs adhered firmly to the porous mesh CGF scaffold, grew vigorously, and stretched sufficiently. In the EDPT group, odontoblasts in the root canal were arranged neatly, and predentin was formed. The odontoblast and dental pulp cell counts in the EDPT group were statistically significant compared to the caries untreated and Ca(OH)2 groups. The hUC-MSCs-CGF could successfully repair necrotic pulp in animals with dental caries.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4012/dmj.2024-007","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

This study investigated the impact of combining human umbilical cord mesenchymal stem cells (hUC-MSCs) with concentrated growth factor (CGF) on regenerating necrotic pulp. Ten-month-old male Bama miniature pigs were divided into control and caries groups. The experimental teeth were randomly divided into three groups: caries untreated, Ca(OH)₂, and engineering dental pulp-like tissue (EDPT). hUC-MSCs and CGF scaffold were combined to construct EDPT, and the histological structure was observed. Odontoblasts and dental pulp cells were counted in each group. The results showed that hUC-MSCs adhered firmly to the porous mesh CGF scaffold, grew vigorously, and stretched sufficiently. In the EDPT group, odontoblasts in the root canal were arranged neatly, and predentin was formed. The odontoblast and dental pulp cell counts in the EDPT group were statistically significant compared to the caries untreated and Ca(OH)₂ groups. The hUC-MSCs-CGF could successfully repair necrotic pulp in animals with dental caries.

查看原文本刊更多论文

人脐带间充质干细胞与浓缩生长因子对修复龋齿引起的坏死牙髓的影响。

本研究探讨了人脐带间充质干细胞（hUC-MSCs）与浓缩生长因子（CGF）结合对坏死牙髓再生的影响。将十个月大的雄性巴马小型猪分为对照组和龋齿组。将 hUC-MSCs 与 CGF 支架结合构建 EDPT，并观察其组织学结构。对每组的牙本质细胞和牙髓细胞进行计数。结果显示，hUC-间充质干细胞能牢固地附着在多孔网状 CGF 支架上，生长旺盛，并能充分伸展。在 EDPT 组中，根管内的牙本质细胞排列整齐，并形成了前牙素。与龋病未处理组和 Ca(OH)2 组相比，EDPT 组的牙本质细胞和牙髓细胞数量具有统计学意义。hUC-间充质干细胞-CGF能成功修复龋齿动物的坏死牙髓。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.