Effects of Adding Tricalcium Silicate Nanoparticles to the Universal G2 Bond Adhesive as Self-Etch Mode on the Shear Bond Strength to the Orthodontic Bracket

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-10-25 DOI:10.1002/cre2.948

Yasir R. Al-Labban, Mehdi Alrubayee, Syed Jaffar Abbas Zaidi, Shakeel Kazmi

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Abstract

Objective

This study investigated the effects of adding tricalcium silicate nanoparticles (TCSNp) to the universal G2 bond adhesive (G2BU) in self-etch (SE) mode on shear bond strength (SBS) to orthodontic brackets, cytotoxicity, and degree of conversion (DC).

Material and Methods

A total of 176 human teeth were divided into four groups based on TCSNp concentration in G2BU adhesive: 0% (control), 1%, 3%, and 5%. The G2BU adhesive consists of a hydrophilic primer (P) and a hydrophobic bonding agent (2B). TCSNp were added to the 2B component by mixing 0.1, 0.3, and 0.5 g of TCSNp with 9.9, 9.7, and 9.5 g of 2B, respectively. SBS was assessed after 24 h of water storage and 5000 thermocycles using a universal testing machine. Cytotoxicity was evaluated using the MTT assay on rat embryo fibroblast cells, and DC was measured using fourier-transform infrared spectroscopy. Statistical analysis included one-way ANOVA and Tukey's post-hoc test, with significance set at p < 0.05.

Results

After 24 h, mean SBS values were 15.58 MPa (control), 13.66 MPa (1% TCSNp), 15.99 MPa (3% TCSNp), and 12.04 MPa (5% TCSNp). After 5000 thermocycles, SBS values decreased to 12.91 MPa (control), 12.42 MPa (1% TCSNp), 11.11 MPa (3% TCSNp), and 10.21 MPa (5% TCSNp). ANOVA showed significant differences between groups (p < 0.05), except between the control and 3% TCSNp groups. Cell viability increased with higher TCSNp concentrations, with significant differences at 72 h between control and 5% TCSNp groups (p = 0.014). Mean DC values were 51.66% (control), 49.33% (1% TCSNp), 49.66% (3% TCSNp), and 48% (5% TCSNp). ANOVA indicated no significant differences between groups.

Conclusions

Adding TCSNp to G2BU in SE mode maintains clinically acceptable SBS levels and enhances cytocompatibility. Higher TCSNp concentrations may reduce SBS and DC slightly. Further studies are needed to evaluate long-term effects.

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在通用 G2 粘接剂中添加纳米硅酸三钙颗粒作为自蚀模式对正畸托槽剪切粘接强度的影响

研究目的本研究探讨了在通用 G2 粘接剂（G2BU）中添加纳米硅酸三钙颗粒（TCSNp），在自酸蚀（SE）模式下对正畸托槽剪切粘接强度（SBS）、细胞毒性和转换度（DC）的影响：根据 G2BU 粘合剂中 TCSNp 的浓度将 176 颗人类牙齿分为四组：0%（对照组）、1%、3% 和 5%。G2BU 粘合剂由亲水性底漆（P）和疏水性粘接剂（2B）组成。将 0.1、0.3 和 0.5 克 TCSNp 分别与 9.9、9.7 和 9.5 克 2B 混合后添加到 2B 组份中。经过 24 小时的水储存和使用万能试验机进行 5000 次热循环后，对 SBS 进行了评估。细胞毒性采用 MTT 法对大鼠胚胎成纤维细胞进行评估，DC 采用傅立叶变换红外光谱法进行测量。统计分析包括单因素方差分析和 Tukey 的事后检验，显著性以 p 为标准：24 小时后，平均 SBS 值分别为 15.58 兆帕（对照组）、13.66 兆帕（1% TCSNp）、15.99 兆帕（3% TCSNp）和 12.04 兆帕（5% TCSNp）。经过 5000 次热循环后，SBS 值分别降至 12.91 兆帕（对照组）、12.42 兆帕（1% TCSNp）、11.11 兆帕（3% TCSNp）和 10.21 兆帕（5% TCSNp）。方差分析显示各组之间存在明显差异（p 结论：TCSNp 和 TCSNp 在 GBS 中的比例为 1:1：在 SE 模式下向 G2BU 中添加 TCSNp 可保持临床可接受的 SBS 水平并增强细胞相容性。较高浓度的 TCSNp 可能会略微降低 SBS 和 DC。需要进一步研究以评估长期效果。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.