Chicken Embryo Fibroblast Viability and Trans-Differentiation Potential for Cultured Meat Production Across Passages.

IF 5.1 2区 生物学 Q2 CELL BIOLOGY
Cells Pub Date : 2024-10-19 DOI:10.3390/cells13201734
So-Hee Kim, Chan-Jin Kim, Eun-Yeong Lee, Young-Hwa Hwang, Seon-Tea Joo
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Abstract

This study was conducted to analyze the viability of primary chicken embryo fibroblasts and the efficiency of adipogenic trans-differentiation for cultured meat production. In isolating chicken embryo fibroblasts (CEFs) from a heterogeneous cell pool containing chicken satellite cells (CSCs), over 90% of CEFs expressed CD29 and vimentin. The analysis of the proliferative capabilities of CEFs revealed no significant differences in EdU-positive cells (%), cumulative cell number, doubling time, and growth rate from passage 1 to passage 9 (p > 0.05). This indicates that CEFs can be isolated by 2 h of pre-plating and survive stably up to passage 9, and that primary fibroblasts can serve as a valuable cell source for the cultured meat industry. Adipogenic trans-differentiation was induced up to passage 9 of CEFs. As passages increased, lipid accumulation and adipocyte size significantly decreased (p < 0.05). The reduced differentiation rate of primary CEFs with increasing passages poses a major challenge to the cost and efficiency of cultured meat production. Thus, effective cell management and the maintenance of cellular characteristics for a long time are crucial for ensuring stable and efficient cultured fat production in the cultured meat industry.

鸡胚胎成纤维细胞在不同阶段培养肉类生产中的活力和转分化潜力。
本研究旨在分析原代鸡胚成纤维细胞的存活率以及用于培养肉类生产的成脂肪转分化效率。从含有鸡卫星细胞(CSCs)的异质细胞池中分离鸡胚成纤维细胞(CEFs)时,90%以上的 CEFs 表达 CD29 和波形蛋白。对 CEFs 增殖能力的分析表明,从第 1 期到第 9 期,CEFs 的 EdU 阳性细胞(%)、累积细胞数、倍增时间和生长率均无显著差异(p > 0.05)。这表明,CEFs 可在预培养 2 小时后分离出来,并稳定存活至第 9 个阶段,原代成纤维细胞可作为培养肉类工业的重要细胞来源。诱导成脂性转分化可持续到 CEFs 第 9 个阶段。随着培养倍数的增加,脂质积累和脂肪细胞体积明显减小(p < 0.05)。原代 CEF 的分化率随着传代的增加而降低,这对培养肉类生产的成本和效率提出了重大挑战。因此,有效的细胞管理和长期保持细胞特性对于确保培养肉工业中稳定高效的培养脂肪生产至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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