Transcriptomic analysis of the gonads of Locusta migratoria (Orthoptera: Acrididae) following infection with Paranosema locustae.

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Xuewei Kong, Xinrui Guo, Jun Lin, Hui Liu, Huihui Zhang, Hongxia Hu, Wangpeng Shi, Rong Ji, Roman Jashenko, Han Wang
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引用次数: 0

Abstract

Paranosema locustae is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of Locusta migratoria males and females infected and uninfected with P. locustae at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein-protein interaction analysis results indicated that L. migratoria regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after P. locustae infection. Immune- and reproductive-related genes in L. migratoria gonadal tissue were screened based on database annotation information and relevant literature. Genes such as Tsf, Hex1, Apolp-III, Serpin, Defense, Hsp70, Hsp90, JHBP, JHE, JHEH1, JHAMT, and VgR play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, Tsf, Hex1, and ApoLp-III were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and P. locustae.

蝗虫感染 Paranosema locustae 后蝗虫性腺的转录组分析。
蝗虫副疟原虫是一种环境友好型寄生捕食者,在蝗虫控制方面具有广阔的应用前景。本研究对感染和未感染蝗蝇寄生虫的雌雄蝗虫在不同发育阶段的性腺组织进行了转录组测序。在雌性卵巢组织转录组中共鉴定出18,635个差异表达基因(DEGs),其中在卵巢破裂后1天观察到的DEGs数量最多(7141个)。在雄性睾丸组织转录组中,共鉴定出 32954 个 DEGs,其中在睾丸凋亡后 9 天观察到的 DEGs 数量最多(11245 个)。维恩分析显示,雌性组中有25个共同的DEGs,雄性组中有205个共同的DEGs。基因本体论和《京都基因和基因组百科全书》分析表明,DEGs主要富集于基础代谢,如氨基酸代谢、碳水化合物代谢、脂质代谢和免疫反应过程。蛋白-蛋白相互作用分析结果表明,在蝗虫感染后的不同发育阶段,褐飞虱调控免疫和生殖相关基因的表达,以满足机体的需求。根据数据库注释信息和相关文献,对移虫生殖腺组织中的免疫和生殖相关基因进行了筛选。Tsf、Hex1、Apolp-III、Serpin、Defense、Hsp70、Hsp90、JHBP、JHE、JHEH1、JHAMT和VgR等基因在性腺组织的免疫反应和生殖平衡中发挥着重要作用。为了验证转录组,研究人员选择了Tsf、Hex1和ApoLp-III,并通过实时定量聚合酶链反应(qRT-PCR)进行了验证。相关分析表明,qRT-PCR表达模式与RNA-Seq结果一致。这些发现有助于进一步了解蝗虫与蝗虫之间的相互作用机制。
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来源期刊
CiteScore
4.00
自引率
0.00%
发文量
160
审稿时长
6-12 weeks
期刊介绍: Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.
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