Xuewei Kong, Xinrui Guo, Jun Lin, Hui Liu, Huihui Zhang, Hongxia Hu, Wangpeng Shi, Rong Ji, Roman Jashenko, Han Wang
{"title":"Transcriptomic analysis of the gonads of <i>Locusta migratoria</i> (Orthoptera: Acrididae) following infection with <i>Paranosema locustae</i>.","authors":"Xuewei Kong, Xinrui Guo, Jun Lin, Hui Liu, Huihui Zhang, Hongxia Hu, Wangpeng Shi, Rong Ji, Roman Jashenko, Han Wang","doi":"10.1017/S0007485324000592","DOIUrl":null,"url":null,"abstract":"<p><p><i>Paranosema locustae</i> is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of <i>Locusta migratoria</i> males and females infected and uninfected with <i>P. locustae</i> at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein-protein interaction analysis results indicated that <i>L. migratoria</i> regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after <i>P. locustae</i> infection. Immune- and reproductive-related genes in <i>L. migratoria</i> gonadal tissue were screened based on database annotation information and relevant literature. Genes such as <i>Tsf</i>, <i>Hex1</i>, <i>Apolp-III</i>, <i>Serpin</i>, <i>Defense</i>, <i>Hsp70</i>, <i>Hsp90</i>, <i>JHBP</i>, <i>JHE</i>, <i>JHEH1</i>, <i>JHAMT</i>, and <i>VgR</i> play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, <i>Tsf</i>, <i>Hex1</i>, and <i>ApoLp-III</i> were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and <i>P. locustae</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0007485324000592","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Paranosema locustae is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of Locusta migratoria males and females infected and uninfected with P. locustae at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein-protein interaction analysis results indicated that L. migratoria regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after P. locustae infection. Immune- and reproductive-related genes in L. migratoria gonadal tissue were screened based on database annotation information and relevant literature. Genes such as Tsf, Hex1, Apolp-III, Serpin, Defense, Hsp70, Hsp90, JHBP, JHE, JHEH1, JHAMT, and VgR play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, Tsf, Hex1, and ApoLp-III were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and P. locustae.
期刊介绍:
Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.