Physiological and transcriptomic analysis reveals the coating of microcapsules embedded with bacteria can enhance wheat salt tolerance.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-25 DOI:10.1186/s12870-024-05718-w

Min Gong, Wei Han, Yawen Jiang, Xi Yang, Jiuxing He, Meng Kong, Qiuyan Huo, Guohua Lv

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引用次数: 0

Abstract

Salt stress is one of the most important abiotic stress factors limiting crop production. Therefore, improving the stress resistance of seeds is very important for crop growth. Our previous studies have shown that using microcapsules encapsulating bacteria (Pontibacter actiniarum DSM 19842) as seed coating for wheat can alleviate salt stress. In this study, the genes and pathways involved in the response of wheat to salt stress were researched further. The results showed that compared with the control, the coating can improve osmotic stress and decrease oxidative damage by increasing the content of proline (29.1%), the activity of superoxide dismutase (SOD) (94.2%), peroxidase (POD) (45.7%) and catalase (CAT) (3.3%), reducing the content of hydrogen peroxide (H₂O₂) (39.8%) and malondialdehyde (MDA) (45.9%). In addition, ribonucleic acid (RNA) sequencing data showed that 7628 differentially expressed genes (DEGs) were identified, and 4426 DEGs up-regulated, 3202 down-regulated in the coated treatment. Many DEGs related to antioxidant enzymes were up-regulated, indicating that coating can promote the expression of antioxidant enzyme-related genes and alleviate oxidative damage under salt stress. The differential gene expression analysis demonstrated up-regulation of 27 genes and down-regulation of 20 genes. Transcription factor families, mostly belonging to bHLH, MYB, B3, NAC, and WRKY. Overall, this seed coating can promote the development of sustainable agriculture in saline soil.

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生理学和转录组分析表明，包埋细菌的微胶囊可以提高小麦的耐盐性。

盐胁迫是限制作物产量的最重要的非生物胁迫因素之一。因此，提高种子的抗逆性对作物生长非常重要。我们之前的研究表明，使用包裹细菌（Pontibacter actiniarum DSM 19842）的微胶囊作为小麦的种子包衣可以缓解盐胁迫。本研究进一步研究了小麦对盐胁迫反应的相关基因和通路。结果表明，与对照组相比，包衣能通过增加脯氨酸含量（29.1%）、超氧化物歧化酶（SOD）活性（94.2%）、过氧化物酶（POD）活性（45.7%）和过氧化氢酶（CAT）活性（3.3%）、降低过氧化氢（H2O2）含量（39.8%）和丙二醛（MDA）含量（45.9%）来改善渗透胁迫和减少氧化损伤。此外，核糖核酸（RNA）测序数据显示，共鉴定出 7628 个差异表达基因（DEGs），其中 4426 个 DEGs 在涂布处理中上调，3202 个下调。许多与抗氧化酶相关的 DEGs 上调，表明包衣能促进抗氧化酶相关基因的表达，减轻盐胁迫下的氧化损伤。差异基因表达分析表明，27 个基因上调，20 个基因下调。转录因子家族大多属于 bHLH、MYB、B3、NAC 和 WRKY。总之，这种种子包衣可以促进盐碱地可持续农业的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.