Niosome-loaded Tet-Amp against S. aureus, K. pneumoniae, and P. aeruginosa.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Elham Bazargan, Fatemeh Ashrafi, Elham Siasi Torbati
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引用次数: 0

Abstract

Biofilm-associated disorders contribute to elevated morbidity and death rates among patients. We propose synthesizing niosomal structures containing the antibiotics tetracycline and ampicillin ((Tet/Amp)-Nio) and investigating their impact on standard strains of S. aureus, K. pneumoniae, and P. aeruginosa. The antibacterial and anti-biofilm effects of synthesized niosomes against standard pathogenic bacterial strains were studied, and also its cytotoxic activity was investigated against human foreskin fibroblast (HFF) cell line. The optimal formulation (F2) had an average particle size of 196.90 ± 4.57 nm, a PDI of 0.223 ± 0.013, a Zeta-potential of -19.25 ± 1.19 mV, a %EE of 70.92 ± 1.75% for Tet and 58.34 ± 1.85% for Amp, and a %Release rate of 49.34 ± 1.78% for Tet and 62.67 ± 1.19% for Amp. The release of Tet and Amp drugs over 48 h was 47% and 61%, respectively, from the (Tet/Amp)-Nio formulation. Also, our findings demonstrated that the Tet/Amp)-Nio have potent antibacterial, anti-biofilm, and lower cytotoxic activity compared to the Tet + Amp. In addition, (Tet/Amp)-Nio can upregulate the expression level of matrix metallopeptidase 2 (MMP2) and matrix metallopeptidase 9 (MMP9) genes, which shows their great activity in the wound healing process. The findings of the current investigation suggest that (Tet/Amp)-Nio enhances its antibacterial and antibiofilm effects against S. aureus, P. aeruginosa, and K. pneumoniae isolates. These formulations may serve as a novel approach for targeted drug delivery.

含 Niosome 的 Tet-Amp 可对抗金黄色葡萄球菌、肺炎双球菌和绿脓杆菌。
生物膜相关疾病导致患者发病率和死亡率升高。我们建议合成含有抗生素四环素和氨苄西林((Tet/Amp)-Nio)的niosomal结构,并研究其对金黄色葡萄球菌、肺炎双球菌和绿脓杆菌标准菌株的影响。研究了合成iosomes 对标准致病细菌菌株的抗菌和抗生物膜作用,还研究了其对人包皮成纤维细胞(HFF)的细胞毒活性。最佳配方(F2)的平均粒径为 196.90 ± 4.57 nm,PDI 为 0.223 ± 0.013,Zeta 电位为 -19.25 ± 1.19 mV,Tet 的释放率为 70.92 ± 1.75%,Amp 的释放率为 58.34 ± 1.85%,Tet 的释放率为 49.34 ± 1.78%,Amp 的释放率为 62.67 ± 1.19%。在(Tet/Amp)-Nio 制剂中,48 小时内 Tet 和 Amp 药物的释放率分别为 47% 和 61%。我们的研究结果还表明,与 Tet + Amp 相比,(Tet/Amp)-Nio 具有较强的抗菌、抗生物膜和较低的细胞毒性活性。此外,(Tet/Amp)-Nio 还能上调基质金属肽酶 2(MMP2)和基质金属肽酶 9(MMP9)基因的表达水平,这表明它们在伤口愈合过程中具有很强的活性。目前的研究结果表明,(Tet/Amp)-Nio 可增强其对金黄色葡萄球菌、铜绿假单胞菌和肺炎双球菌分离物的抗菌和抗生物膜作用。这些制剂可作为靶向给药的一种新方法。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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