Genomic analysis of Salmonella Heidelberg isolated from the Brazilian poultry farms.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Emanuela Mendes Cardoso, Juliano De Dea Lindner, Fabienne Antunes Ferreira
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引用次数: 0

Abstract

The rapid expansion of broiler chicken production in Brazil has presented significant sanitation challenges within the poultry industry. Among these challenges, Salmonella enterica subsp. enterica serotype Heidelberg stands as a contributor to global salmonellosis outbreaks. This study analyzed 13 draft genomes of Salmonella Heidelberg isolated from the pre-slaughter broiler chickens farms in Brazil. By conducting in silico analysis of these genomes, the study investigated genome similarity based on single nucleotide polymorphisms (SNPs) and identified genes encoding resistance to antimicrobials, sanitizers, and virulence factors. Furthermore, mobile genetic elements (MGE) were identified to assess their potential role in propagating genes through horizontal gene transfer. A risk classification was also applied based on the resistomes. The genomes revealed a high prevalence of genes conferring resistance to aminoglycosides, fosfomycin, sulfonamides, tetracycline, and genes linked to quaternary ammonium resistance. The study also uncovered six Salmonella pathogenicity islands (SPI) and over 100 genes encoding virulence factors. The association of MGE with antibiotic-resistant genes sul2 and blaCMY-2 raised concerns about the potential transfer to other bacteria, posing a substantial risk for spreading resistance mechanisms according to established risk protocols. Additionally, SNP analysis indicated close phylogenetic relationships among some isolates, suggesting a common origin. This study enhances our understanding of Salmonella Heidelberg strains by identifying key risk factors for transmission and revealing the association between resistance genes and MGEs. This insight provides a foundation for developing and implementing effective control, monitoring, and treatment strategies in the poultry industry.

从巴西家禽养殖场分离出的海德堡沙门氏菌的基因组分析。
巴西肉鸡生产的快速扩张给家禽业带来了巨大的卫生挑战。在这些挑战中,肠炎沙门氏菌亚种海德堡血清型是导致全球沙门氏菌病爆发的原因之一。本研究分析了从巴西宰前肉鸡养殖场分离出的 13 个海德堡沙门氏菌基因组草案。通过对这些基因组进行硅分析,研究人员根据单核苷酸多态性(SNPs)调查了基因组的相似性,并确定了编码抗菌素、消毒剂和毒力因子抗性的基因。此外,还确定了移动遗传因子(MGE),以评估它们在通过水平基因转移传播基因方面的潜在作用。还根据抗药性基因组进行了风险分类。基因组显示,对氨基糖苷类、磷霉素、磺胺类、四环素类药物产生抗性的基因以及与季铵抗性相关的基因非常普遍。研究还发现了六个沙门氏菌致病性岛(SPI)和 100 多个编码毒力因子的基因。MGE 与抗生素耐药基因 sul2 和 blaCMY-2 的关联引起了人们对其可能转移到其他细菌的担忧,根据既定的风险规程,这构成了耐药机制扩散的巨大风险。此外,SNP 分析表明一些分离物之间存在密切的系统发育关系,表明它们有共同的起源。这项研究通过确定传播的关键风险因素和揭示抗性基因与 MGEs 之间的关联,加深了我们对海德堡沙门氏菌菌株的了解。这一见解为家禽业制定和实施有效的控制、监测和治疗策略奠定了基础。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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