First report on genetic characterization of egg drop syndrome 1976 virus in Egypt.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Emad Al-Ebshahy, Mohammed AboElkhair, Awad Shehata, Emad Elgendy
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Abstract

Since its first description in 1991 in Egypt, egg drop syndrome 1976 (EDS-76) virus has received a little attention as a potential cause for the drop in egg production as well as the reduction in egg quality. To date, no studies have been carried out to describe the genetic characteristics of the circulating field EDS-76 virus strains. Thus, the present study was attempted to estimate the emergence of EDS-76 virus in layer flocks and to determine the genetic diversity between the field strains and the vaccine strain 127. During 2022, a total of 5 apparently healthy backyard layer flocks were investigated for the presence of EDS-76 virus infection following complaints of sudden drop in egg production (25-30%), accompanied by high incidence of eggshell defects. EDS-76 virus DNA was detected in the oviduct samples of 4 (80%) flocks by polymerase chain reaction (PCR) assay targeting the hexon gene of the viral capsid. Attempts of viral isolation in duck embryo revealed no embryonic mortality, however, the allantoic fluids of inoculated eggs exhibited a sustained increase in the hemagglutinating (HA) activity throughout three consecutive passages. The obtained strain, designated BH-1, was characterized on the basis of partial hexon gene sequence analysis (GenBank accession number OR531368). The BH-1 strain displayed 99.6% nucleotide identity with the vaccine strain 127. However, amino acid alignments with the vaccine strain 127 revealed that the BH-1 strain carried 5 non-synonymous mutations. In addition, two of these mutations were incorporated into the hexon hypervariable regions (HVRs), which are strictly responsible for eliciting serotype-specific neutralizing antibodies. In conclusion, the present study represents a starting point for genetic characterization of EDS-76 virus in Egypt and highlights the importance for continuous monitoring and characterization of the circulating field EDS-76 virus strains, in order to determine the proper control strategy.

首次报告埃及 1976 年蛋下降综合征病毒的遗传特征。
自 1991 年首次在埃及被描述以来,蛋鸡产蛋下降综合症 1976(EDS-76)病毒作为产蛋下降和蛋品质量下降的潜在原因受到了关注。迄今为止,还没有研究对田间流行的 EDS-76 病毒株的遗传特征进行描述。因此,本研究试图估算 EDS-76 病毒在蛋鸡群中的出现情况,并确定田间毒株与 127 疫苗毒株之间的遗传多样性。2022年期间,在接到产蛋量突然下降(25%-30%)并伴有高蛋壳缺陷发生率的投诉后,共对5个表面健康的散养蛋鸡群进行了调查,以确定是否存在EDS-76病毒感染。通过聚合酶链式反应(PCR)检测,在 4 个鸡群(80%)的输卵管样本中检测到了 EDS-76 病毒的 DNA,检测的目标是病毒外壳的 hexon 基因。在鸭胚胎中分离病毒的尝试未发现胚胎死亡现象,但接种鸭蛋的尿囊液在连续三个传代过程中显示血凝素(HA)活性持续上升。根据部分 hexon 基因序列分析(GenBank 编号 OR531368),获得的菌株被命名为 BH-1。BH-1 株与疫苗株 127 的核苷酸同一性为 99.6%。然而,与疫苗株 127 的氨基酸比对结果显示,BH-1 株携带 5 个非同义突变。此外,其中两个突变被整合到了己子超变异区(HVRs),而HVRs严格负责激发血清型特异性中和抗体。总之,本研究为埃及 EDS-76 病毒的遗传特征描述提供了一个起点,并强调了持续监测和描述田间流行的 EDS-76 病毒毒株以确定适当的控制策略的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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