Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Marta Rybska, Marek Skrzypski, Maria Billert, Tatiana Wojciechowicz, Anna Łukomska, Piotr Pawlak, Tomasz Nowak, Karolina Pusiak, Barbara Wąsowska
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引用次数: 0

Abstract

Background: Nesfatin-1 is a neuropeptide that regulates the hypothalamic-pituitary-gonadal axis and may play a role in uterus function. It is co-expressed with other peptides, such as phoenixin, which can influence sex hormone secretion. Our previous research has confirmed that phoenixin-14 is involved in the development of cystic endometrial hyperplasia (CEH) and pyometra in dogs. Therefore, based on the similarities and interactions between these neuropeptides, we hypothesized that nesfatin-1 might also regulate the reproductive system in dogs. This study aimed to determine the expression of nesfatin-1 and its interaction with phoenixin-14 in dogs with CEH or pyometra compared to healthy females, and concerning animals' body condition score (BCS 4-5/9 vs. BCS > 5/9).

Results: The analysis of nesfatin-1 in the uterus of bitches consisted of qPCR, western blot and immunofluorescence assays, and ELISAs. The results showed significantly higher nesfatin-1 encoding gene, nucleobindin-2 mRNA (Nucb2) and nesfatin-1 protein expression in overweight females and those suffering from CEH or pyometra compared to healthy animals. The immunoreactivity of nesfatin-1 was elevated in the uteri of bitches with higher BCS > 5/9. Moreover, nesfatin-1 blood concentrations increased in all examined overweight bitches. In the case of phoenixin signals, we found opposite results, regardless of the female body condition score.

Conclusion: The etiology of CEH and pyometra are not fully known, although we have expanded the level of knowledge with respect to the possible interaction of nesfatin-1 and phoenixin in female dogs' uteri. They interact oppositely. With increasing female body weight, the expression of nesfatin-1 in the uterus and its peripheral blood concentration increased. However, for female dogs affected by CEH and pyometra, a decreased level of phoenixin-14, irrespective of their body condition score is characteristic. This knowledge could be crucial in the development of biomarkers for these conditions, which may lead to earlier recognition.

囊性子宫内膜增生症和子宫脓肿雌性犬的 Nesfatin-1 表达和血浆浓度及其与凤凰素-14 的可能相互作用。
背景:Nesfatin-1 是一种调节下丘脑-垂体-性腺轴的神经肽,可能在子宫功能中发挥作用。它与凤凰素等其他多肽共同表达,可影响性激素分泌。我们之前的研究证实,凤凰素-14 参与了狗的子宫内膜囊性增生症(CEH)和子宫脓肿的发病。因此,基于这些神经肽之间的相似性和相互作用,我们推测nesfatin-1也可能调控狗的生殖系统。本研究旨在确定与健康雌性犬相比,患有CEH或子宫脓肿的犬体内nesfatin-1的表达及其与凤凰素-14的相互作用,以及动物的体况评分(BCS 4-5/9 vs. BCS > 5/9):结果:对母犬子宫中内司蛋白-1的分析包括qPCR、Western印迹和免疫荧光检测以及ELISA。结果表明,与健康动物相比,超重雌性动物和患有 CEH 或脓子宫的雌性动物的 nesfatin-1 编码基因、nucleobindin-2 mRNA (Nucb2) 和 nesfatin-1 蛋白表达量明显较高。BCS>5/9的母犬子宫内的nesfatin-1免疫活性升高。此外,在所有受检的超重母畜中,nesfatin-1的血药浓度都有所升高。在凤蝶素信号方面,我们发现了与雌性体况评分无关的相反结果:结论:虽然我们对母犬子宫中内司蛋白-1和凤凰素之间可能存在的相互作用有了更多的了解,但CEH和子宫脓肿的病因还不完全清楚。它们的相互作用是相反的。随着雌犬体重的增加,nesfatin-1在子宫中的表达量及其外周血浓度也随之增加。然而,对于受 CEH 和子宫脓肿影响的雌性犬,无论其身体状况评分如何,凤凰素-14 的水平都会下降。这些知识对于开发这些病症的生物标记物至关重要,可能有助于更早地识别这些病症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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