Is castration leading to biological aging in dogs? Assessment of lipid peroxidation, inflammation, telomere length, mitochondrial DNA copy number, and expression of telomerase and age-related genes.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2024-10-24 DOI:10.1186/s12917-024-04337-9

Hossein Hassanpour, Moosa Javdani, Zahra Changaniyan-Khorasgani, Elnaz Rezazadeh, Reza Jalali, Marzieh Mojtahed

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引用次数: 0

Abstract

Background: Biological aging is a complex process influenced by various factors, including reproductive status and castration. This study aimed to evaluate the impact of castration on biological aging in dogs.

Method: Fifteen male crossbred dogs were randomly divided into a sham-operation control group (n = 5) and a castrated group (n = 10). Blood samples were collected at weeks 0, 4, 8, 12, 16, and 18 post-surgery. Malondialdehyde (MDA as indicator of Lipid peroxidation), C-reactive protein (as an indicator of inflammation), telomere length, mitochondrial DNA (mtDNA) copy number, and the expression of age-related (P16, P21, TBX2) and telomerase-related (TERT) genes were assessed in blood samples.

Results: Plasma MDA levels were higher in the control group at weeks 16 and 18, while CRP levels were higher only at week 18. Telomere length and mtDNA copy number were lower in the control group at week 18. Gene expression analysis showed that P16 was lower in the control group at weeks 8 and 12, P21 and TERT were lower at weeks 16 and 18, and TBX2 was lower at weeks 16 and 18. The TBX2/P16 ratio was lower in the control group at weeks 16 and 18 but higher at week 12, while the TBX2/P21 ratio did not differ between groups.

Conclusion: Castration appears to have a protective effect against biological aging in dogs, as evidenced by lower lipid peroxidation, inflammation, and age-related changes in telomere length, mtDNA copy number, and gene expression.

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阉割会导致狗的生物衰老吗？评估脂质过氧化、炎症、端粒长度、线粒体 DNA 拷贝数以及端粒酶和年龄相关基因的表达。

背景：生物衰老是一个复杂的过程，受各种因素的影响，包括生殖状况和阉割。本研究旨在评估阉割对犬生物衰老的影响：方法：将 15 只雄性杂交犬随机分为假手术对照组（5 只）和阉割组（10 只）。在手术后第 0、4、8、12、16 和 18 周采集血液样本。对血液样本中的丙二醛（MDA，脂质过氧化指标）、C反应蛋白（炎症指标）、端粒长度、线粒体DNA（mtDNA）拷贝数、年龄相关基因（P16、P21、TBX2）和端粒相关基因（TERT）的表达进行评估：结果：对照组的血浆 MDA 水平在第 16 周和第 18 周较高，而 CRP 水平仅在第 18 周较高。在第 18 周时，对照组的端粒长度和 mtDNA 拷贝数较低。基因表达分析表明，对照组的 P16 在第 8 周和第 12 周较低，P21 和 TERT 在第 16 周和第 18 周较低，TBX2 在第 16 周和第 18 周较低。对照组的TBX2/P16比值在第16周和第18周时较低，但在第12周时较高，而TBX2/P21比值在组间没有差异：结论：阉割似乎对狗的生物衰老有保护作用，这体现在较低的脂质过氧化、炎症以及端粒长度、mtDNA拷贝数和基因表达中与年龄相关的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.