Chemical and Biological Characterization of Metabolites from Silene viridiflora Using Mass Spectrometric and Cell-Based Assays.

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2024-10-11 DOI:10.3390/biom14101285

Nilufar Z Mamadalieva, Alexey Koval, Maksud M Dusmuratov, Hidayat Hussain, Vladimir L Katanaev

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Abstract

A comprehensive metabolite profiling of the medicinal plant Silene viridiflora using an UHPLC-ESI-MS/MS method is described for the first time. A total of 71 compounds were identified and annotated, the most common of which were flavonoids, triterpene glycosides, and ecdysteroids. The three major compounds schaftoside, 26-hydroxyecdysone, and silviridoside can be chosen as the markers for the assessment of the quality of S. viridiflora preparations. The methanol extract and a variety of metabolites identified in S. viridiflora were screened for their cytotoxic and Wnt pathway-inhibiting activities against triple-negative breast cancer (TNBC), the deadliest form of cancer in women. 2-Deoxy-20-hydroxyecdysone with submicromolar IC₅₀ was identified as a result. The structure-activity relationship derived from the data from the in vitro proliferation assay showed that the hydroxyl group present at position C-2 of steroid core reduces the ecdysteroids' cytotoxicity against cancer cells.

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利用质谱法和基于细胞的检测方法对 Silene viridiflora 的代谢物进行化学和生物学表征。

本研究首次采用超高效液相色谱-电喷雾电离质谱/质谱法对药用植物西莲花（Silene viridiflora）进行了全面的代谢物分析。共鉴定并注释了 71 种化合物，其中最常见的是黄酮类化合物、三萜苷类化合物和蜕皮甾类化合物。三种主要化合物蛇床子甙、26-羟基蜕皮激素和蛇床子甙可作为评估蛇床子制剂质量的指标。针对三阴性乳腺癌（TNBC）--女性中最致命的癌症形式--的细胞毒性和 Wnt 通路抑制活性，筛选了甲醇提取物和在 S. viridiflora 中鉴定出的多种代谢物。结果发现，2-脱氧-20-羟基蜕皮激素的 IC50 值达到亚摩尔级。根据体外增殖试验数据得出的结构-活性关系表明，类固醇核心 C-2 位上的羟基降低了蜕皮激素对癌细胞的细胞毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.