MATE transporter OsMATE2 mediates root growth, grain size and weight by interacting with Mn-SOD and PABP in rice

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2024-10-18 DOI:10.1016/j.bbrc.2024.150821

Xiao Yan-jia , Yu Si-si , Zheng Yan-mei , Wang Xin-yue , Zeng Xiao-yu , Deng Lan-lan , Li Han-ren , Zhu Yong-sheng , Cai Qiu-hua , Xie Hua-an , Zhang Jian-fu

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引用次数: 0

Abstract

Multidrug and toxic compound extrusion proteins (MATE) can transport small organic molecules in and out of cells and participate in detoxification, nutrient absorption, disease resistance and plant development processes. These compounds are widely distributed in plants. However, the mechanism by which MATE affects grain development remains elusive. In this study, we studied a MATE transporter, OsMATE2, which localized on the membrane. The CRISPR-Cas9 (CR) knockout line of OsMATE2 presented obvious decreases in grain weight. In addition, root development was also affected. Two proteins that interact with OsMATE2, namely, manganese-superoxide dismutase (Mn-SOD) and poly(A)-binding protein (PABP), were identified from a screening of yeast library. The results were validated through yeast two-hybrid and bimolecular fluorescence complementation experiments. The CRISPR-Cas9 (CR) knockout lines of Mn-SOD and PABP presented increased grain size and weight. Our findings demonstrated that OsMATE2 interacts with Mn-SOD and PABP to regulate grain development in rice.

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MATE转运体OsMATE2通过与水稻中的Mn-SOD和PABP相互作用，介导根的生长、粒度和重量。

多药和有毒化合物挤出蛋白（MATE）可将小分子有机物运入和运出细胞，并参与解毒、营养吸收、抗病和植物发育过程。这些化合物广泛分布于植物体内。然而，MATE 影响谷物发育的机理仍不清楚。在这项研究中，我们研究了一种定位于膜上的 MATE 转运体 OsMATE2。CRISPR-Cas9（CR）敲除OsMATE2的品系会导致谷粒重量明显下降。此外，根的发育也受到影响。通过筛选酵母文库，发现了两种与OsMATE2相互作用的蛋白质，即锰-超氧化物歧化酶（Mn-SOD）和多聚（A）结合蛋白（PABP）。这些结果通过酵母双杂交和双分子荧光互补实验得到了验证。Mn-SOD和PABP的CRISPR-Cas9（CR）基因敲除株系表现出更大的粒径和重量。我们的研究结果表明，OsMATE2与Mn-SOD和PABP相互作用，调控水稻谷粒的发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics