Protein Modifications During Early Embryo Development

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2024-10-26 DOI:10.1111/aji.70007

Le Zhang, Yanbing Zhang, Hailong Sun

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引用次数: 0

Abstract

Background

Infertility is a global reproductive health burden. Assisted reproductive technologies (ARTs) have been widely used to help patients become pregnant. Few embryos develop to the blastocyst stage with ARTs, leading to relatively low live birth rates. Protein modifications play crucial roles in nearly every aspect of cell biology, including reproductive processes. The aim of this study was to explore the characteristics of protein modifications during embryonic development.

Methods

Proteomic data from humans and mice were acquired from the integrated proteome resources (iProX) of ProteomeXchange (PXD024267) and a tandem mass tag (TMT)-mass spectrometry dataset. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied for functional annotation. Protein–protein interactions (PPIs) of the modification-related genes were revealed by the STRING database. Modified proteins during mouse embryogenesis were visualized through heatmaps of hierarchically clustering using k-means.

Results

We identified modification-related proteins in human embryo development and characterized them through heatmaps, GO analysis, KEGG analysis, and PPI network analysis. We found that the 4-cell stage to the 8-cell stage might be the demarcation period for modification-related protein expression patterns during embryo development. Using quantitative mass spectrometry, we elucidated the methylation, acetylation, and ubiquitination events that occur during mouse embryogenesis to validate our findings in human embryonic development to some extent.

Conclusions

The results of our study suggest that the posttranslational modifications (PTMs) of human preimplantation embryos might exhibit the same trends as those in mice to exert synergistic and fine-tuned regulatory effects during embryonic development.

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胚胎早期发育过程中的蛋白质修饰

背景：不孕症是全球生殖健康的一大负担。辅助生殖技术（ART）已被广泛用于帮助患者怀孕。使用辅助生殖技术的胚胎很少发育到囊胚阶段，导致活产率相对较低。蛋白质修饰在细胞生物学的几乎每个方面都起着至关重要的作用，包括生殖过程。本研究旨在探索胚胎发育过程中蛋白质修饰的特点：方法：从 ProteomeXchange（PXD024267）的综合蛋白质组资源（iProX）和串联质量标签（TMT）质谱数据集中获得了人类和小鼠的蛋白质组数据。基因本体（GO）分析和京都基因组百科全书（KEGG）分析被用于功能注释。STRING数据库揭示了修饰相关基因的蛋白-蛋白相互作用（PPIs）。小鼠胚胎发生过程中的修饰蛋白通过使用k-means进行分层聚类的热图可视化：我们发现了人类胚胎发育过程中与修饰相关的蛋白质，并通过热图、GO分析、KEGG分析和PPI网络分析对其进行了表征。我们发现，4细胞期到8细胞期可能是胚胎发育过程中修饰相关蛋白表达模式的分界期。通过定量质谱分析，我们阐明了小鼠胚胎发育过程中发生的甲基化、乙酰化和泛素化事件，从而在一定程度上验证了我们在人类胚胎发育过程中的发现：我们的研究结果表明，人类植入前胚胎的翻译后修饰（PTMs）可能会表现出与小鼠相同的趋势，在胚胎发育过程中发挥协同和微调的调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.