Dynamic susceptibility contrast-enhanced MRI with USPIO in evaluating angiogenesis of the peri-infarction zones in subacute ischemic stroke in a permanent middle cerebral artery occlusion rat model.

IF 1.1 4区医学 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Acta radiologica Pub Date : 2024-12-01 Epub Date: 2024-10-24 DOI:10.1177/02841851241290646

Yuanchao Li, Fang Lu, Cheng Zhang, Huihui Xu, Shuohui Yang

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引用次数: 0

Abstract

Background: Dynamic susceptibility contrast-enhanced magnetic resonance imaging (DSC-MRI) can reflect the angiogenesis of ischemic stroke.

Purpose: To investigate the value of DSC-MRI with ultrasmall superparamagnetic particles of iron oxides (USPIO) in evaluating angiogenesis in the peri-infarction zones in subacute ischemic stroke in a permanent middle cerebral artery occlusion (pMCAO) rat model.

Material and methods: A total of 21 Sprague-Dawley rats were randomly divided into the pMCAO and sham operation groups. Every rat in each group underwent DSC-MRI with USPIO at 3, 5, and 7 days. DSC-MRI parameters of the relative cerebral blood volume (rCBV), relative cerebral blood flow (rCBF), relative mean transit time (rMTT), and relative time to peak (rTTP) were measured, calculated, and compared among the different times. Sequential correlations were analyzed among the histopathological indexes with the microvascular density (MVD) and percentage of vascular area (%VA), the serum factors with vascular endothelial growth factor (VEGF), vascular cell adhesion molecule 1 (VCAM-1), and perfusion parameters, respectively.

Results: The rCBV and rCBF in the peri-infarction area of pMCAO rats were significantly higher on day 7 than on day 3, whereas no significant changes in rMTT and rTTP were observed at 3, 5, and 7 days. Significantly positive correlations were found between rCBV and MVD, %VA, VEGF, VCAM-1, between rCBF and MVD, %VA, VEGF, and VCAM-1 at 3, 5, and 7 days in the pMCAO group.

Conclusion: The rCBV and rCBF deriving from USPIO-DSC may be potentially useful for evaluating the angiogenesis of the peri-infarction zones in the subacute phase of ischemic stroke.

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用 USPIO 动态感性对比增强磁共振成像评估永久性大脑中动脉闭塞大鼠模型亚急性缺血性中风梗死周围区域的血管生成。

背景：目的：在永久性大脑中动脉闭塞（pMCAO）大鼠模型中，研究超小型超顺磁性氧化铁颗粒（USPIO）DSC-MRI在评估亚急性缺血性卒中梗死周围血管生成中的价值：将 21 只 Sprague-Dawley 大鼠随机分为 pMCAO 组和假手术组。每组的每只大鼠都在 3 天、5 天和 7 天时接受了 USPIO 的 DSC-MRI 检查。DSC-MRI参数包括相对脑血容量（rCBV）、相对脑血流量（rCBF）、相对平均通过时间（rMTT）和相对达峰时间（rTTP），这些参数在不同时间进行测量、计算和比较。分析了组织病理学指标与微血管密度（MVD）和血管面积百分比（%VA）之间的序列相关性，血清因子与血管内皮生长因子（VEGF）、血管细胞粘附分子1（VCAM-1）和灌注参数之间的序列相关性：pMCAO大鼠梗死周围区域的rCBV和rCBF在第7天显著高于第3天，而rMTT和rTTP在第3、5和7天均无显著变化。在 pMCAO 组中，rCBV 与 MVD、%VA、VEGF 和 VCAM-1 之间，rCBF 与 MVD、%VA、VEGF 和 VCAM-1 之间在 3、5 和 7 天内均呈显著正相关：结论：USPIO-DSC 得出的 rCBV 和 rCBF 可能有助于评估缺血性中风亚急性阶段梗死周围区域的血管生成情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta radiologica 医学-核医学

CiteScore

2.70

自引率

0.00%

发文量

170

审稿时长

3-8 weeks

期刊介绍： Acta Radiologica publishes articles on all aspects of radiology, from clinical radiology to experimental work. It is known for articles based on experimental work and contrast media research, giving priority to scientific original papers. The distinguished international editorial board also invite review articles, short communications and technical and instrumental notes.