A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species using Ag@carbon dots composites

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-10-25 DOI:10.1007/s00604-024-06783-6

Ruilin Xiao, Haixia Liang, Baohua Tian, Xinxin Li, Tingshan Song

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引用次数: 0

Abstract

A novel fluorescent sensor based on silver nanoparticle-carbon dot composites (Ag@CDs) has been developed for the rapid and quantitative detection of aquatic bacteria. The sensor operates on the principle of plasmon-enhanced resonance energy transfer, where the fluorescence of CDs is quenched by Ag nanoparticles and restored upon bacterial interaction due to the generation of reactive oxygen species. The Ag@CDs exhibit a linear response to bacterial concentration over the range 7 × 10⁴ ~ 4 × 10⁷ CFU·mL⁻¹, with a low detection limit of 4 × 10⁴ CFU·mL⁻¹. The fluorescence recovery is rapid, reaching maximum intensity within 5 min. The method demonstrates high selectivity, with minimal interference from common ions and compounds found in municipal and industrial wastewater. The Ag@CDs-based 96-well plate assay for quantitative measurement of bacteria was developed. The assay’s performance was further validated through the analysis of real water samples, showing a recovery of 94.0 ~ 102% for domestic wastewater and 97.6 ~ 106% for industrial wastewater. Also, Ag@CDs-based test strips assay for semi-quantitation were developed for rapid in-field aquatic bacteria detection. Ag@CDs can be conveniently integrated into 96-well plates and test strips, providing rapid on-site aquatic bacteria testing.

Graphical abstract

查看原文本刊更多论文

基于细菌活性氧的 Ag@carbon dots 复合材料快速定量检测水生细菌的荧光传感器。

我们开发了一种基于银纳米粒子-碳点复合材料（Ag@CDs）的新型荧光传感器，用于快速定量检测水生细菌。该传感器的工作原理是质子增强共振能量转移，银纳米粒子淬灭碳点的荧光，在与细菌相互作用时，由于活性氧的产生，碳点的荧光恢复。Ag@CDs 在 7 × 104 ~ 4 × 107 CFU-mL-1 的范围内对细菌浓度呈线性响应，检测限低至 4 × 104 CFU-mL-1。荧光恢复迅速，5 分钟内即可达到最大强度。该方法选择性高，受城市和工业废水中常见离子和化合物的干扰极小。开发了基于 Ag@CDs 的 96 孔板细菌定量检测方法。通过对真实水样的分析，进一步验证了该检测方法的性能，结果表明生活废水的回收率为 94.0 ~ 102%，工业废水的回收率为 97.6 ~ 106%。此外，还开发了基于 Ag@CDs 的半定量试纸，用于现场水生细菌的快速检测。Ag@CDs 可方便地集成到 96 孔板和试纸中，提供现场水生细菌快速检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.