Chronic heat stress upregulates pyruvate metabolic process and gluconeogenesis but downregulates immune responses in Sahiwal cattle.

IF 3 3区 地球科学 Q2 BIOPHYSICS
Pradyut Das, Rani Alex, Gopal Ramdasji Gowane, Vikas Vohra, Dipankar Paul, Kashif Dawood Khan, Amritanshu Upadhyay, Sachinandan De, Ashutosh Ludri
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Abstract

Climate change and growing population and their strain on animal production are the impending challenges that the developing countries, like India, need to tackle in the coming days. This study aimed to detect and analyze the uncharacterized variation in the gene expression patterns with the change of condition, from thermoneutral to chronic hot-humid, in the Sahiwal cattle, one of the best breeds of milk-producing cattle in India, known for being heat-tolerant. Using RNA-Seq analysis on peripheral blood mononuclear cells (PBMCs), 4021 differentially expressed mRNAs (2772 upregulated, 1249 downregulated) and 1303 differentially expressed long non-coding RNAs (769 upregulated, 534 downregulated) were identified, with the thresholds of false discovery rate < 0.05 and|log2(fold change)| > 2. Significantly (p-adjusted < 0.05) overrepresented Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathways were analyzed, revealing upregulation of processes like pyruvate metabolic process, gluconeogenesis, ion transmembrane transport, neuropeptide signaling pathway, and animal organ development, with genes like SHH, GRK1, CHRM3, CAMK2A, and HSPB7 were upregulated, while translation and immune responses, with genes like RPS3, EEF1A1, TNF, BoLA-DRB3, and UBB were downregulated. Analysis of cis-mRNAs of DE-lncRNAs showed presence of both up- and down-regulated cis-mRNAs for both up- and down-regulated lncRNAs indicating existence of positive and negative regulation of mRNA expression by lncRNAs. Managemental nudges that decrease metabolic heat generation, like betaine and chromium supplementation, and increase heat dissipation, like microenvironment cooling, should be utilized. This study highlights the role of pyruvate metabolism and gluconeogenesis in coping up with heat stress and offers an improved understanding of the heat stress response of Sahiwal cattle along with the genes and pathways responsible for it.

慢性热应激会上调丙酮酸代谢过程和葡萄糖生成,但会下调萨希瓦尔牛的免疫反应。
气候变化和人口增长及其对畜牧业生产的影响是印度等发展中国家在未来需要应对的挑战。萨希瓦尔牛是印度产奶量最好的牛种之一,以耐热著称,本研究旨在检测和分析萨希瓦尔牛基因表达模式随温度变化(从中性到长期湿热)而发生的未定性变化。通过对外周血单核细胞(PBMCs)进行 RNA-Seq 分析,发现了 4021 个差异表达的 mRNAs(2772 个上调,1249 个下调)和 1303 个差异表达的长非编码 RNAs(769 个上调,534 个下调),假发现率为 2(折合变化)| > 2。显著(p
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来源期刊
CiteScore
6.40
自引率
9.40%
发文量
183
审稿时长
1 months
期刊介绍: The Journal publishes original research papers, review articles and short communications on studies examining the interactions between living organisms and factors of the natural and artificial atmospheric environment. Living organisms extend from single cell organisms, to plants and animals, including humans. The atmospheric environment includes climate and weather, electromagnetic radiation, and chemical and biological pollutants. The journal embraces basic and applied research and practical aspects such as living conditions, agriculture, forestry, and health. The journal is published for the International Society of Biometeorology, and most membership categories include a subscription to the Journal.
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