Curcumin-loaded zein nanoparticles: A quality by design approach for enhanced drug delivery and cytotoxicity against cancer cells.

IF 5.4 2区 医学 Q1 BIOPHYSICS
Jayalakshmi Cs, Mohamed Haider, Mutasem Rawas-Qalaji, Pallab Sanpui
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Abstract

Zein, a maize protein, has been explored for constructing potential biomaterial due to its hydrophobic nature, self-assembly capability, and biocompatibility. In its nanoparticulate form, zein is a promising material for drug delivery applications, particularly in cancer treatment. Despite the importance of colloidal stability for effective drug delivery, systematic studies investigating the effect of various surface modifying agents (MAs) on the zein nanoparticles (ZNPs)-based formulations are limited. This study employs quality-by-design (QbD) approach to optimize curcumin-loaded ZNPs, enhancing colloidal stability, size, and drug-encapsulation efficiency using different MAs for potential cancer therapy. Gum arabic (GA) emerged as the optimal stabilizer, with GA-stabilized curcumin-loaded ZNPs (GA-Cur-ZNPs) achieving a particle size of 184.8 ± 2.85 nm, zeta potential of -23.4 ± 0.56 mV and 87.1 ±1.55 % drug encapsulation efficiency, along with excellent colloidal stability over two months. The optimal formulation also demonstrated sustained release of Cur over 72 h. GA-Cur-ZNPs demonstrated lower IC50 values and higher anti-proliferative effects on three different cancer cell lines compared to the free drug, while also exhibiting superior intracellular uptake. With negligible toxicity to human dermal fibroblast cells, the optimized Cur-GA-ZNPs show promise for safe and effective killing of cancer cells.

姜黄素负载的玉米蛋白纳米颗粒:通过设计提高质量,增强对癌细胞的药物输送和细胞毒性。
玉米蛋白因其疏水性质、自组装能力和生物相容性,已被用于构建潜在的生物材料。玉米蛋白的纳米颗粒形式是一种很有前景的给药材料,尤其是在癌症治疗方面。尽管胶体稳定性对有效给药非常重要,但有关各种表面修饰剂(MAs)对基于玉米蛋白纳米颗粒(ZNPs)的制剂的影响的系统研究却很有限。本研究采用质量源于设计(QbD)的方法优化姜黄素负载的 ZNPs,利用不同的 MAs 提高胶体稳定性、尺寸和药物包囊效率,以用于潜在的癌症治疗。阿拉伯胶(GA)成为最佳稳定剂,经 GA 稳定的姜黄素负载 ZNPs(GA-Cur-ZNPs)的粒径为 184.8 ± 2.85 nm,zeta 电位为 -23.4 ± 0.56 mV,药物包封效率为 87.1 ± 1.55 %,并且在两个月内具有极佳的胶体稳定性。最佳配方还能在 72 小时内持续释放 Cur。与游离药物相比,GA-Cur-ZNPs 的 IC50 值更低,对三种不同癌细胞系的抗增殖作用更强,同时还表现出卓越的细胞内吸收能力。经过优化的 Cur-GA-ZNPs 对人类真皮成纤维细胞的毒性可以忽略不计,因此有望安全有效地杀死癌细胞。
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来源期刊
Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces 生物-材料科学:生物材料
CiteScore
11.10
自引率
3.40%
发文量
730
审稿时长
42 days
期刊介绍: Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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