Developing oxygen vacancy-rich CuMn2O4/carbon dots dual-function nanozymes via Chan-Lam coupling reaction for the colorimetric/fluorescent determination of D-penicillamine.

IF 10.7 1区 生物学 Q1 BIOPHYSICS
Biosensors and Bioelectronics Pub Date : 2025-01-01 Epub Date: 2024-10-19 DOI:10.1016/j.bios.2024.116864
Min Feng, Xiaodan Zhang, Yuming Huang
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引用次数: 0

Abstract

Defect engineering is a promising approach to construct high performance nanozymes due to its ability to regulate their physical and chemical properties. However, how to construct defects to improve the activity of nanozymes remains a challenge. Herein, for the first time, the Chan-Lam coupling reaction is used to construct the oxygen vacancy (OV)-rich CuMn2O4/carbon dots (CDs) (OV-CuMn2O4/CDs) dual-function nanozymes with fluorescent (FL) and oxidase-like properties, via regulating the low-valent metal ions (Cu+ and Mn2+) and Ov contents in the spinel CuMn2O4 and in-situ growth of β-cyclodextrin (β-CD)-derived CDs. Expectedly, relative to CuMn2O4, the OV-CuMn2O4/CDs exhibited 35.8%, 8.5%, and 14.6% rise in the contents of Cu+, Mn2+ and Ov, respectively. Abundant Ov provides more O2 adsorption/activation sites, and the charge transfer between Ov and metal atoms increases the charge density around metal atoms. This produces more low-valent metals (like Cu+ and Mn2+) to promote the electron transfer from metal to O atoms and O-O bond cleavage. Thus, the oxidase-like activity of OV-CuMn2O4/CDs is 4.1 times that of CuMn2O4. Also, the in-situ growth of β-CD-derived carbon dots on CuMn2O4 endows OV-CuMn2O4/CDs selective target recognition. Thus, a sensitive and selective colorimetric and fluorescence dual-mode method was established for determining D-penicillamine (D-PA), with the limit of detection of 0.25 and 0.048 μM, respectively. The method was applied to D-PA determination in real samples. This work demonstrates the Chan-Lam coupling reaction can be used to construct high performance nanozymes for developing dual-mode sensor for the detection of targets.

通过 Chan-Lam 偶联反应开发富氧空位铜锰氧化物/碳点双功能纳米酶,用于 D-青霉胺的比色/荧光测定。
由于缺陷工程能够调节纳米酶的物理和化学性质,因此是构建高性能纳米酶的一种前景广阔的方法。然而,如何构建缺陷以提高纳米酶的活性仍然是一个挑战。本文首次利用 Chan-Lam 偶联反应构建了富氧空位(OV)CuMn2O4/碳点(CDs)(OV-CuMn2O4/CDs)双功能纳米酶,具有荧光(FL)和类氧化酶特性、通过调节尖晶石 CuMn2O4 中的低价金属离子(Cu+ 和 Mn2+)和 Ov 含量,以及原位生长 β-环糊精(β-CD)衍生的 CD。与 CuMn2O4 相比,OV-CuMn2O4/CDs 中 Cu+、Mn2+ 和 Ov 的含量分别增加了 35.8%、8.5% 和 14.6%。丰富的 Ov 提供了更多的氧气吸附/活化位点,Ov 和金属原子之间的电荷转移增加了金属原子周围的电荷密度。这就产生了更多的低价金属(如 Cu+ 和 Mn2+),以促进从金属到 O 原子的电子转移和 O-O 键的裂解。因此,OV-CuMn2O4/CDs 的类氧化酶活性是 CuMn2O4 的 4.1 倍。此外,β-CD 衍生碳点在 CuMn2O4 上的原位生长赋予了 OV-CuMn2O4/CDs 选择性目标识别能力。因此,建立了一种灵敏、选择性的比色和荧光双模式方法来测定 D-青霉胺(D-PA),其检出限分别为 0.25 μM 和 0.048 μM。该方法被应用于实际样品中 D-PA 的测定。这项工作证明了 Chan-Lam 偶联反应可用于构建高性能纳米酶,以开发检测目标物的双模式传感器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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