Elevated CO2 Concentration Extends Reproductive Growth Period and Enhances Carbon Metabolism in Wheat Exposed to Increased Temperature.

Abstract

Both elevated atmospheric CO₂ concentration ([CO₂]) and increased temperature exert notable influences on wheat (Triticum aestivum L.) growth and productivity when examined individually. Nevertheless, limited research comprehensively investigates the combined effects of both factors. Winter wheat was grown in environment-controlled chambers under two concentrations of CO₂ (ambient CO₂ concentration and ambient CO₂ concentration plus 200 µmol mol^-1) and two levels of temperature (ambient temperature and ambient temperature plus 2°C). The phenology, photosynthesis, carbohydrate and nitrogen metabolism, yield and quality responses of wheat were investigated. Elevated [CO₂] did not counteract warming-induced shortening of wheat phenological period but prolonged grain filling. Even though photosynthetic adaptation occurred during the reproductive growth period, elevated [CO₂] still significantly enhanced carbohydrate accumulation under warming, particularly at the grain filling stage, thereby increasing yield by 20.1% compared with the ambient control. However, elevated [CO₂] inhibited nitrogen assimilation at the grain filling stage under increased temperature by downregulating the expression levels of TaNR, TaNIR, TaGS1 and TaGOGAT and reducing glutamine synthetase activity, which directly led to a significant decrease of 19.4% in grain protein content relative to the ambient control. These findings suggest that elevated [CO₂] will likely increase yield but decrease grain nutritional quality for wheat under future global warming scenarios.