NpCIPK6–NpSnRK1 module facilitates inter-subgeneric hybridization barriers in water lily (nymphaea) by reducing abscisic acid content

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Research Pub Date : 2024-10-23 DOI:10.1093/hr/uhae289

Ping Zhou, Jingwen Li, Huiyan Jiang, Zhijuan Yang, Chunqing Sun, Hongyan Wang, Qun Su, Qijiang Jin, Yanjie Wang, Yingchun Xu

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引用次数: 0

Abstract

Pre-fertilization hybridization barriers are the main causes of inter-subgeneric hybridization challenges in water lily. However, the mechanism underlying low compatibility between pollen and stigma of water lily remains unclear. This study demonstrates that CBL-interacting protein kinase 6 (CIPK6) responded to the signaling exchange between incompatible pollen and stigma through interactions with SNF1-related kinase 1 (SnRK1) and promotes the accumulation of SnRK1 protein. Activated SnRK1 interacted with 9-cis-epoxycarotenoid dioxygenase 2 (NCED2) to promote its degradation, thereby inhibiting abscisic acid (ABA) synthesis. A decrease in ABA content in the stigma impaired the ABA-mediated removal of reactive oxygen species (ROS), ultimately resulting in the rejection of the incompatible pollen by the stigma. Our results highlight the essential role of the NpCIPK6–NpSnRK1–NpNCED2 module in conferring inter-subgeneric hybridization barriers in water lily by interfering with ABA synthesis and promoting ROS accumulation. This study offers valuable mechanistic insights into cellular signaling and reproductive barriers in water lily as well as across other biological contexts.

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NpCIPK6-NpSnRK1 模块通过降低脱落酸含量促进睡莲亚属间杂交障碍的形成

受精前杂交障碍是睡莲亚属间杂交难题的主要原因。然而，睡莲花粉与柱头之间低相容性的机制仍不清楚。本研究表明，CBL-互作蛋白激酶6（CIPK6）通过与SNF1相关激酶1（SnRK1）相互作用，对不相容花粉和柱头之间的信号交换做出反应，并促进SnRK1蛋白的积累。活化的 SnRK1 与 9-顺式环氧类胡萝卜素二氧酶 2（NCED2）相互作用，促进其降解，从而抑制脱落酸（ABA）的合成。柱头中 ABA 含量的减少损害了 ABA 介导的活性氧（ROS）清除，最终导致不相容花粉被柱头排斥。我们的研究结果突出表明，NpCIPK6-NpSnRK1-NpNCED2 模块通过干扰 ABA 合成和促进 ROS 积累，在赋予睡莲亚属间杂交障碍方面起着至关重要的作用。这项研究为睡莲以及其他生物环境中的细胞信号传导和繁殖障碍提供了宝贵的机理见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.