Mechanistic insights on stabilization and destabilization effect of ionic liquids on type I collagen fibrils

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2024-10-16 DOI:10.1016/j.jmbbm.2024.106772

Kuntala Banerjee , Christina Mathew , Chandrasekar Inbasekar , Nishter Nishad Fathima

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引用次数: 0

Abstract

Tuned assembly of collagen has tremendous applications in the field of biomedical and tissue engineering owing to its targeted biological functionalities. In this study, ionic liquids choline dihydrogen citrate (CDHC) and diethyl methyl ammonium methane sulfonate (AMS) have been used to regulate the self-assembly of collagen at its physiological pH by probing the assembled systems at certain concentration ratios of ionic liquids and the systems were studied using various characterization methods. Due to interaction with collagen, choline dihydrogen citrate causes delay in the collagen fibrillisation process showing no binding interactions with collagen. In contrast, diethyl methyl ammonium methane sulfonate shows crosslinking effect on collagen fibrillisation due to the electrostatic interaction with the tetrahedral hydration shell of collagen moieties. From rheological studies it was observed that the AMS treated collagen fibril at 1:1 % (w/v) has highest linear viscoelastic range, this can bear the stress under high strain compare to native collagen fibril as well as all CDHC composites. For a sustainable biomaterial or bio-scaffold, mechanical property plays pivotal role on it and from our experimental analysis we found certain composites of ionic liquid treated collagen fibrillar assembly which may act as a sustainable biomaterial or bio-scaffold. It was also evolved that, how the structure-function relationship of ionic force modulated fibrillar assembly controlling the mechanical properties of the tuned system. This self-assembled, ionic-liquid treated collagen-fibrillar system would accelerate various force modulated fibrillar network study, for mimicking the ECM and tissue engineering application.

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离子液体对 I 型胶原纤维的稳定和失稳效应的机理研究。

由于胶原蛋白具有针对性的生物功能，因此调整胶原蛋白的组装在生物医学和组织工程领域有着巨大的应用前景。本研究利用离子液体柠檬酸二氢胆碱（CDHC）和甲烷磺酸二乙基甲基铵（AMS）来调节胶原蛋白在生理 pH 值下的自组装，在离子液体达到一定浓度比时对组装系统进行探测，并使用各种表征方法对系统进行研究。由于与胶原蛋白的相互作用，柠檬酸二氢胆碱会导致胶原蛋白纤维化过程延迟，显示出与胶原蛋白没有结合相互作用。与此相反，甲基磺酸二乙基甲铵由于与胶原蛋白分子的四面体水合壳发生静电作用，对胶原蛋白的纤维化产生了交联作用。流变学研究表明，经 AMS 处理的 1:1 %（w/v）胶原纤维具有最高的线性粘弹性范围，与原生胶原纤维和所有 CDHC 复合材料相比，它可以承受高应变下的应力。对于可持续生物材料或生物支架来说，机械性能起着至关重要的作用。通过实验分析，我们发现某些经离子液体处理的胶原纤维组件复合材料可用作可持续生物材料或生物支架。我们还发现，离子力的结构-功能关系如何调节纤维组装，从而控制调谐系统的机械性能。这种经离子液体处理的自组装胶原纤维系统将加速各种力调制纤维网络的研究，以模拟 ECM 和组织工程应用。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.