A responsive nanoprobe for ratiometric florescence detection of hydroxyl radicals in macrophage polarization†

IF 6.1 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Mazen Alanazi, Miaomiao Wu, Jiaxi Yong, Zexi Zhang, Huayue Zhang, Dihua Tian and Run Zhang
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Abstract

Quantification of hydroxyl radicals (˙OH), one form of reactive oxygen species (ROS), plays critical roles in early diagnosis and treatment monitoring of various diseases. In this work, we report the development of a responsive nanoprobe for ratiometric fluorescence detection and imaging of ˙OH in macrophage polarization. The nanoprobe, BSA-CCA@LDH-SRB, was designed and prepared using coumarin 3-carboxylic acid (CCA) as the sensing unit for ˙OH, and sulforhodamine B (SRB) loaded on layered double hydroxide (LDH) served as the fluorescent reference component. The coupling of CCA to bovine serum albumin (BSA) and the loading of BSA–CCA on the surface of LDH enabled the nanoprobe for fluorescence detection of ˙OH with high sensitivity and minimal interference from other biomolecules, ions, and ROS. The emission of the prepared BSA-CCA@LDH-SRB at 444 nm emerged and the intensity was increased according to the concentration of ˙OH, while the emission at 580 nm was maintained, allowing the nanoprobe for ratiometric fluorescence (F444/580) detection of ˙OH. Loading of the BSA protein on the LDH surface and the biocompatibility and colloidal stability of the LDH-based fluorescent nanoprobe were further improved, facilitating the detection of ˙OH generation in macrophage polarization stimulated by both biomolecules and physical ultrasound irradiation. This study thus offers a new nanoprobe as the tool for investigating ˙OH evolutions, advancing the biomedical investigations of macrophage polarization associated inflammation.

Abstract Image

用于巨噬细胞极化过程中羟基自由基比率荧光检测的响应式纳米探针。
羟基自由基(˙OH)是活性氧(ROS)的一种形式,其定量在各种疾病的早期诊断和治疗监测中起着至关重要的作用。在这项工作中,我们报告了一种用于巨噬细胞极化过程中˙OH 的比率荧光检测和成像的响应性纳米探针的开发情况。该纳米探针名为 BSA-CCA@LDH-SRB,以香豆素-3-羧酸(CCA)作为˙OH 的传感单元,以负载在层状双氢氧化物(LDH)上的磺基罗丹明 B(SRB)作为荧光参比组分。将 CCA 与牛血清白蛋白(BSA)偶联,并在 LDH 表面负载 BSA-CCA,使纳米探针能够高灵敏度地荧光检测˙OH,并将其他生物大分子、离子和 ROS 的干扰降至最低。制备的 BSA-CCA@LDH-SRB 在 444 nm 处出现发射,且发射强度随˙OH 浓度的增加而增加,而在 580 nm 处的发射则保持不变,因此该纳米探针可以对˙OH 进行比率荧光检测(F444/580)。在 LDH 表面载入 BSA 蛋白,进一步提高了基于 LDH 的荧光纳米探针的生物相容性和胶体稳定性,有助于检测生物大分子和物理超声照射刺激巨噬细胞极化过程中˙OH 的产生。因此,该研究提供了一种新的纳米探针作为研究˙OH 演变的工具,从而推进了与炎症相关的巨噬细胞极化的生物医学研究。
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来源期刊
Journal of Materials Chemistry B
Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.30%
发文量
866
期刊介绍: Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive: Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices
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