Bed bugs, Cimex lectularius: Undercover agents in forensic investigations.

Kelly A Meiklejohn, Coby Schal, Khalid M Lodhi
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Abstract

Insects have long played a role in forensic investigations and can be used to estimate minimum time since death, corpse translocation, and link an individual to a crime scene. Bed bugs (Cimex lectularius) are wingless ectoparasitic insects of potential forensic utility, given that all mobile life stages feed on vertebrate blood. Successful profiling of autosomal short tandem repeats (STRs) from human DNA isolated from bed bugs has been previously reported. This proof-of-concept study looked to expand this work and determine any possible limitations of using bed bugs for both rapid stain identification (RSID™) for human blood and Y-STR profiling. To achieve this, bed bugs were fed either human male only or human pooled (female:male) blood for 30 min and subsequently collected at 12-h intervals up to 108 h post-blood meal (PBM). RSID™ blood testing was successful from the bed bug carcass remaining after DNA isolation, regardless of blood meal type and time of collection PBM. Complete Y-STR profiles were generated from bed bugs <60 h PBM. As the time PBM increased, DNA quantity decreased, while the degradation index increased. Collection of bed bugs at a crime scene could provide a valuable source of human blood for Y STR profiling and be used to link an individual to a crime scene or for potential male suspect exclusion. Future studies should look to replicate the results of this proof-of-concept study with larger numbers of bed bugs, more diverse blood donors, and additional STR profiling kits.

臭虫,Cimex lectularius:法医调查中的卧底。
长期以来,昆虫在法医调查中一直扮演着重要角色,可用于估算死亡后的最短时间、尸体转移以及将个体与犯罪现场联系起来。臭虫(Cimex lectularius)是一种无翅的外寄生昆虫,其所有移动生命阶段都以脊椎动物的血液为食,因此具有潜在的法医学用途。以前曾有报道称,从臭虫体内分离出的人类 DNA 成功分析出常染色体短串联重复序列 (STR)。这项概念验证研究旨在扩展这项工作,并确定使用臭虫进行人类血液快速染色鉴定(RSID™)和 Y-STR 分析可能存在的局限性。为此,研究人员给臭虫喂食人类雄性血液或人类混合(雌性:雄性)血液 30 分钟,然后每隔 12 小时收集一次,直至血餐后 108 小时(PBM)。无论血餐类型和 PBM 采集时间如何,RSID™ 血液检测都能从 DNA 分离后剩余的臭虫尸体中成功完成。从臭虫身上生成了完整的 Y-STR 图谱
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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