Evaluation of Minimum-to-Severe Global and Macrovesicular Steatosis in Human Liver Specimens: A Portable Ambient Light-Compatible Spectroscopic Probe

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2024-10-13 DOI:10.1002/jbio.202400292

Hao Guo, Ashley E. Stueck, Jason B. Doppenberg, Yun Suk Chae, Alexey B. Tikhomirov, Haishan Zeng, Marten A. Engelse, Boris L. Gala-Lopez, Anita Mahadevan-Jansen, Ian P. J. Alwayn, Andrea K. Locke, Kevin C. Hewitt

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引用次数: 0

Abstract

Background and Aims

Hepatic steatosis (HS), particularly macrovesicular steatosis (MaS), influences transplant outcomes. Accurate assessment of MaS is crucial for graft selection. While traditional assessment methods have limitations, non-invasive spectroscopic techniques like Raman and reflectance spectroscopy offer promise. This study aimed to evaluate the efficacy of a portable ambient light-compatible spectroscopic system in assessing global HS and MaS in human liver specimens.

Methods

A two-stage approach was employed on thawed snap-frozen human liver specimens under ambient room light: biochemical validation involving a comparison of fat content from Raman and reflectance intensities with triglyceride (TG) quantifications and histopathological validation, contrasting Raman-derived fat content with evaluations by an expert pathologist and a “Positive Pixel Count” algorithm. Raman and reflectance intensities were combined to discern significant (≥ 10%) discrepancies in global HS and MaS.

Results

The initial set of 16 specimens showed a positive correlation between Raman and reflectance-derived fat content and TG quantifications. The Raman system effectively differentiated minimum-to-severe global and macrovesicular steatosis in the subsequent 66 specimens. A dual-variable prediction algorithm was developed, effectively classifying significant discrepancies (> 10%) between algorithm-estimated global HS and pathologist-estimated MaS.

Conclusion

Our study established the viability and reliability of a portable spectroscopic system for non-invasive HS and MaS assessment in human liver specimens. The compatibility with ambient light conditions and the ability to address limitations of previous methods marks a significant advancement in this field. By offering promising differentiation between global HS and MaS, our system introduces an innovative approach to real-time and quantitative donor HS assessments. The proposed method holds the promise of refining donor liver assessment during liver recovery and ultimately enhancing transplantation outcomes.

Abstract Image

查看原文本刊更多论文

评估人体肝脏标本中从轻度到重度的全局性和大泡性脂肪变性：与环境光兼容的便携式光谱探针

背景和目的：肝脏脂肪变性（HS），尤其是大泡性脂肪变性（MaS）会影响移植结果。准确评估大泡性脂肪变性对移植选择至关重要。虽然传统的评估方法有其局限性，但拉曼光谱和反射光谱等非侵入性光谱技术却大有可为。本研究旨在评估一种与环境光兼容的便携式光谱系统在评估人体肝脏标本的全局HS和MaS方面的功效：在室内环境光下对解冻的速冻人体肝脏标本采用了两个阶段的方法：生化验证，包括将拉曼和反射强度得出的脂肪含量与甘油三酯 (TG) 定量进行比较；组织病理学验证，将拉曼得出的脂肪含量与病理专家的评估和 "正像素计数 "算法进行对比。将拉曼强度和反射强度结合起来，以鉴别全局 HS 和 MaS 的显著差异（≥ 10%）：最初的 16 个标本显示，拉曼和反射衍生的脂肪含量和总热量定量之间存在正相关。在随后的 66 例标本中，拉曼系统有效地区分了从轻度到重度的全局性和大泡性脂肪变性。我们还开发了一种双变量预测算法，可有效区分算法估算的整体 HS 与病理学家估算的大泡性脂肪变性之间的显著差异（> 10%）：我们的研究证实了便携式光谱系统在人体肝脏标本中进行无创 HS 和 MaS 评估的可行性和可靠性。与环境光条件的兼容性以及解决以往方法局限性的能力标志着这一领域的重大进步。我们的系统可以很好地区分全局 HS 和 MaS，为实时和定量评估供体 HS 引入了一种创新方法。所提出的方法有望完善肝脏恢复期间的供体肝脏评估，并最终提高移植效果。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.