Comparison of behavioral responses, respiratory metabolism-related enzyme activities, and metabolomics of the juvenile Chinese mitten crab Eriocheir sinensis with different tolerance to air exposure

Abstract

Air exposure is a common stressor for Chinese mitten crab (Eriocheir sinensis) during rearing and transport, and air exposure tolerance can serve as a crucial indicator for assessing the quality of juvenile E. sinensis. In this study, juvenile E. sinensis were divided into two groups based on their behavioral responses: Group S, which exhibited strong tolerance to air exposure, and Group W, which exhibited weak tolerance. Immersed crabs, not exposed to air, served as a control group (Group C). Whole body morphological characteristics and enzyme activities related to respiratory metabolism in the hemolymph and anterior gills were compared among the three groups. Non-targeted LC-MS metabolomic analysis was conducted on anterior gills. The results showed that, independent of developmental stage, crabs that were larger and had higher condition factor were more tolerant to air exposure. Additionally, compared to Group W, air exposure had a relatively small effect on glycolysis and anaerobic respiratory metabolic processes in the hemolymph and anterior gills of Group S. In response to air exposure, E. sinensis experienced increased energy demand, and switched from aerobic to anaerobic respiration to increase energy supply. Simultaneously, air exposure induced oxidative stress in the hemolymph and anterior gills. This study enhances our understanding of the response mechanism of E. sinensis to air exposure and provides a theoretical reference for the identification of high-quality juvenile E. sinensis.